ICC/IF analysis of L929 Cells using GTX79355 TIMP2 antibody [F27 P3 A4]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

ICC/IF analysis of Neuro-2a Cells using GTX79355 TIMP2 antibody [F27 P3 A4]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

ICC/IF analysis of HeLa cells using GTX79355 TIMP2 antibody [F27 P3 A4]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

IHC-P analysis of human pancreas tissue using GTX79355 TIMP2 antibody [F27 P3 A4].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:500

ICC/IF analysis of HeLa cells using GTX79355 TIMP2 antibody [F27 P3 A4]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

IHC-P analysis of mouse pancreas tissue using GTX79355 TIMP2 antibody [F27 P3 A4].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100