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TIMP2 antibody [F27 P3 A4]

Anti-TIMP2 antibody [F27 P3 A4] used in IHC (Paraffin sections) (IHC-P). GTX79355
Anti-TIMP2 antibody [F27 P3 A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79355
Anti-TIMP2 antibody [F27 P3 A4] used in IHC (Paraffin sections) (IHC-P). GTX79355
Anti-TIMP2 antibody [F27 P3 A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79355
Anti-TIMP2 antibody [F27 P3 A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79355
Anti-TIMP2 antibody [F27 P3 A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79355

Cat. No. GTX79355

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 F27 P3 A4

Isotype

 IgG2

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all TIMP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF 1-2 μg/ml
IHC-P 1:50-1:500
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide corresponding to residues D(56) S G N D I Y G N P I(66) of mouse TIMP-2.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_11171602

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tissue inhibitor of metalloproteinase 2 , D11Bwg1104e , Timp-2

Background

Tissue inhibitor of metalloproteinases-1 (TIMP-1) and Tissue inhibitor of metalloproteinases-2 (TIMP-2) are inhibitory enzymes of matrix metalloproteinase family. TIMP-1 forms a complex of 1:1 stoichiometry with activated interstitial collagenase, activated stromelysin, active form of 72 kD Type IV collagenase (MMP-2) and latent and active forms 92 kD Type IV collagenase (MMP-9). TIMPs inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Database

Research Area

DATA IMAGES

Anti-TIMP2 antibody [F27 P3 A4] used in IHC (Paraffin sections) (IHC-P). GTX79355

GTX79355 IHC-P Image

IHC-P analysis of mouse pancreas tissue using GTX79355 TIMP2 antibody [F27 P3 A4].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

Anti-TIMP2 antibody [F27 P3 A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79355

GTX79355 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX79355 TIMP2 antibody [F27 P3 A4]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

Anti-TIMP2 antibody [F27 P3 A4] used in IHC (Paraffin sections) (IHC-P). GTX79355

GTX79355 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX79355 TIMP2 antibody [F27 P3 A4].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:500

Anti-TIMP2 antibody [F27 P3 A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79355

GTX79355 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX79355 TIMP2 antibody [F27 P3 A4]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-TIMP2 antibody [F27 P3 A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79355

GTX79355 ICC/IF Image

ICC/IF analysis of Neuro-2a Cells using GTX79355 TIMP2 antibody [F27 P3 A4]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-TIMP2 antibody [F27 P3 A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79355

GTX79355 ICC/IF Image

ICC/IF analysis of L929 Cells using GTX79355 TIMP2 antibody [F27 P3 A4]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399