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TIMP3 antibody [N1C3]

Anti-TIMP3 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100742
Anti-TIMP3 antibody [N1C3] used in Western Blot (WB). GTX100742

Cat No. GTX100742

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

mouse kidney

Predict Reactivity

Human, Rabbit, Sheep, Bovine, Dog, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.51 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TIMP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241393

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

TIMP metallopeptidase inhibitor 3 , HSMRK222 , K222 , K222TA2 , SFD

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TIMP3 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100742

GTX100742 ICC/IF Image

TIMP3 antibody [N1C3] detects TIMP3 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TIMP3 stained by TIMP3 antibody [N1C3] (GTX100742) diluted at 1:500.
Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-TIMP3 antibody [N1C3] used in Western Blot (WB). GTX100742

GTX100742 WB Image

TIMP3 antibody detects TIMP3 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with TIMP3 antibody (GTX100742) diluted at 1:500.

REFERENCE

There are currently no references for TIMP3 antibody [N1C3] (GTX100742). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for TIMP3 antibody [N1C3] (GTX100742). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169