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TIMP3 antibody [N1C3]

Anti-TIMP3 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100742
Anti-TIMP3 antibody [N1C3] used in Western Blot (WB). GTX100742

Cat No. GTX100742

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

mouse kidney

Predict Reactivity

Human, Rabbit, Sheep, Bovine, Dog, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.51 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TIMP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

TIMP metallopeptidase inhibitor 3 , HSMRK222 , K222 , K222TA2 , SFD

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-TIMP3 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100742

GTX100742 ICC/IF Image

TIMP3 antibody [N1C3] detects TIMP3 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TIMP3 stained by TIMP3 antibody [N1C3] (GTX100742) diluted at 1:500.
Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-TIMP3 antibody [N1C3] used in Western Blot (WB). GTX100742

GTX100742 WB Image

TIMP3 antibody detects TIMP3 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with TIMP3 antibody (GTX100742) diluted at 1:500.

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169