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TIP30 antibody

Anti-TIP30 antibody used in Western Blot (WB). GTX100119
Anti-TIP30 antibody used in Immunoprecipitation (IP). GTX100119
Anti-TIP30 antibody used in IHC (Paraffin sections) (IHC-P). GTX100119
Anti-TIP30 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100119
Anti-TIP30 antibody used in IHC (Paraffin sections) (IHC-P). GTX100119
Anti-TIP30 antibody used in IHC (Paraffin sections) (IHC-P). GTX100119

Cat. No. GTX100119

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all TIP30 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

A431 , HeLa

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human TIP30 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11165752

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HIV-1 Tat interactive protein 2 , CC3 , SDR44U1 , TIP30

Cellular Localization

Cytoplasm , Nucleus envelope

Background

Oxidoreductase required for tumor suppression. NAPDH-bound form inhibits nuclear import by competing with nuclear import substrates for binding to a subset of nuclear transport receptors. May act as a redox sensor linked to transcription through regulation of nuclear import. Isoform 1 is a metastasis suppressor with proapoptotic as well as antiangiogenic properties. Isoform 2 has an antiapoptotic effect.

Database

Research Area

DATA IMAGES

Anti-TIP30 antibody used in Western Blot (WB). GTX100119

GTX100119 WB Image

Sample (30 ug of whole cell lysate)
A: A431
B: HeLa
12% SDS PAGE
GTX100119 diluted at 1:5000

Anti-TIP30 antibody used in Immunoprecipitation (IP). GTX100119

GTX100119 IP Image

TIP30 antibody immunoprecipitates TIP30 protein in IP experiments. IP Sample: hela whole cell lysate/extract A. 40 μg hela whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of TIP30 protein by 2 μg of TIP30 antibody (GTX100119) 10% SDS-PAGE The immunoprecipitated TIP30 protein was detected by TIP30 antibody (GTX100119) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent

Anti-TIP30 antibody used in IHC (Paraffin sections) (IHC-P). GTX100119

GTX100119 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using TIP30(GTX100119) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TIP30 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100119

GTX100119 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using TIP30(GTX100119) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-TIP30 antibody used in IHC (Paraffin sections) (IHC-P). GTX100119

GTX100119 IHC-P Image

TIP30 antibody detects TIP30 protein at cytoplasm in mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
TIP30 antibody (GTX100119) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TIP30 antibody used in IHC (Paraffin sections) (IHC-P). GTX100119

GTX100119 IHC-P Image

TIP30 antibody detects TIP30 protein on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
TIP30 antibody (GTX100119) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 399
$ 169