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TIP30 antibody

Anti-TIP30 antibody used in Immunoprecipitation (IP). GTX100119
Anti-TIP30 antibody used in Western Blot (WB). GTX100119
Anti-TIP30 antibody used in IHC (Paraffin sections) (IHC-P). GTX100119
Anti-TIP30 antibody used in IHC (Paraffin sections) (IHC-P). GTX100119
Anti-TIP30 antibody used in IHC (Paraffin sections) (IHC-P). GTX100119
Anti-TIP30 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100119

Cat No. GTX100119

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

A431 , HeLa

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human TIP30 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HIV-1 Tat interactive protein 2,CC3,SDR44U1,TIP30

Cellular Localization

Cytoplasm , Nucleus envelope

Background

Oxidoreductase required for tumor suppression. NAPDH-bound form inhibits nuclear import by competing with nuclear import substrates for binding to a subset of nuclear transport receptors. May act as a redox sensor linked to transcription through regulation of nuclear import. Isoform 1 is a metastasis suppressor with proapoptotic as well as antiangiogenic properties. Isoform 2 has an antiapoptotic effect.

Database

Research Area

DATA IMAGES
Anti-TIP30 antibody used in Immunoprecipitation (IP). GTX100119

GTX100119 IP Image

TIP30 antibody immunoprecipitates TIP30 protein in IP experiments. IP Sample: hela whole cell lysate/extract A. 40 μg hela whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of TIP30 protein by 2 μg of TIP30 antibody (GTX100119) 10% SDS-PAGE The immunoprecipitated TIP30 protein was detected by TIP30 antibody (GTX100119) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent

Anti-TIP30 antibody used in Western Blot (WB). GTX100119

GTX100119 WB Image

Sample (30 ug of whole cell lysate)
A: A431
B: HeLa
12% SDS PAGE
GTX100119 diluted at 1:5000

Anti-TIP30 antibody used in IHC (Paraffin sections) (IHC-P). GTX100119

GTX100119 IHC-P Image

TIP30 antibody detects TIP30 protein at cytoplasm in mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
TIP30 antibody (GTX100119) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TIP30 antibody used in IHC (Paraffin sections) (IHC-P). GTX100119

GTX100119 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using TIP30(GTX100119) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TIP30 antibody used in IHC (Paraffin sections) (IHC-P). GTX100119

GTX100119 IHC-P Image

TIP30 antibody detects TIP30 protein on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
TIP30 antibody (GTX100119) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TIP30 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100119

GTX100119 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using TIP30(GTX100119) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169