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TIP60 antibody

Orthogonal Overexpression
Anti-TIP60 antibody used in Western Blot (WB). GTX112197
Anti-TIP60 antibody used in Western Blot (WB). GTX112197
Anti-TIP60 antibody used in Western Blot (WB). GTX112197
Anti-TIP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX112197

Cat. No. GTX112197

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all TIP60 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

Tip60 transfected 293T, Jurkat nuclear extract

Predict Reactivity

Rat, Bovine, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TIP60. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950610

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lysine acetyltransferase 5 , ESA1 , HTATIP , HTATIP1 , PLIP , TIP , TIP60 , ZC2HC5 , cPLA2

Cellular Localization

Nucleus , nucleolus , Cytoplasm , perinuclear region

Background

The protein encoded by this gene belongs to the MYST family of histone acetyl transferases (HATs) and was originally isolated as an HIV-1 TAT-interactive protein. HATs play important roles in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins. This protein is a histone acetylase that has a role in DNA repair and apoptosis and is thought to play an important role in signal transduction. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TIP60 antibody used in Western Blot (WB). GTX112197

GTX112197 WB Image

Overexpression

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TIP60 antibody (GTX112197) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TIP60 antibody used in Western Blot (WB). GTX112197

GTX112197 WB Image

Orthogonal

Jurkat whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TIP60 antibody (GTX112197) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TIP60 antibody used in Western Blot (WB). GTX112197

GTX112197 WB Image

Orthogonal Overexpression

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TIP60 antibody (GTX112197) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TIP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX112197

GTX112197 IHC-P Image

TIP60 antibody detects TIP60 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
TIP60 stained by TIP60 antibody (GTX112197) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Zhang X et al. J Virol 2024; 98 (3) : e0169523 NS1-mediated enhancement of MVC transcription and replication promoted by KAT5/H4K12ac.
Idrissou M et al. Cancer Genomics Proteomics 2020; 17 (6) : 687-694 TIP60/P400/H4K12ac Plays a Role as a Heterochromatin Back-up Skeleton in Breast Cancer.
Bulut-Karslioglu A et al. Cell Stem Cell 2018; 22 (3) : 369-383.e8 The Transcriptionally Permissive Chromatin State of Embryonic Stem Cells Is Acutely Tuned to Translational Output.
Ashraf W et al. J Exp Clin Cancer Res 2017; 36 (1) : 188 Interaction of the epigenetic integrator UHRF1 with the MYST domain of TIP60 inside the cell.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169