APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
Tip60 transfected 293T , mouse brain , Jurkat nuclear extract
Predict Reactivity
Rat, Bovine, Pig, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.81 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human TIP60. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950610
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
lysine acetyltransferase 5 , ESA1 , HTATIP , HTATIP1 , PLIP , TIP , TIP60 , ZC2HC5 , cPLA2
Cellular Localization
Nucleus , nucleolus , Cytoplasm , perinuclear region
Background
The protein encoded by this gene belongs to the MYST family of histone acetyl transferases (HATs) and was originally isolated as an HIV-1 TAT-interactive protein. HATs play important roles in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins. This protein is a histone acetylase that has a role in DNA repair and apoptosis and is thought to play an important role in signal transduction. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX112197 WB Image
Sample (30 μg of whole cell lysate)
A: Non-transfected 293T lysate
B: Tip60 transfected 293T lysate
GTX112197 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX112197 WB Image
Jurkat whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TIP60 antibody (GTX112197) diluted at 1:1000.
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GTX112197 WB Image
TIP60 antibody detects KAT5 protein by western blot analysis.
A. 50 μg mouse brain lysate/extract
7.5% SDS-PAGE
TIP60 antibody (GTX112197) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
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