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TIP60 antibody

Anti-TIP60 antibody used in Western Blot (WB). GTX112197
Anti-TIP60 antibody used in Western Blot (WB). GTX112197
Anti-TIP60 antibody used in Western Blot (WB). GTX112197

Cat No. GTX112197

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

Jurkat , Jurkat nuclear extract , mouse brain , Tip60 transfected 293T lysate

Predict Reactivity

Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TIP60. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950610

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

lysine acetyltransferase 5 , ESA1 , HTATIP , HTATIP1 , PLIP , TIP , TIP60 , ZC2HC5 , cPLA2

Cellular Localization

Nucleus , nucleolus , Cytoplasm , perinuclear region

Background

The protein encoded by this gene belongs to the MYST family of histone acetyl transferases (HATs) and was originally isolated as an HIV-1 TAT-interactive protein. HATs play important roles in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins. This protein is a histone acetylase that has a role in DNA repair and apoptosis and is thought to play an important role in signal transduction. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TIP60 antibody used in Western Blot (WB). GTX112197

GTX112197 WB Image

Sample (30 μg of whole cell lysate)
A: Non-transfected 293T lysate
B: Tip60 transfected 293T lysate
GTX112197 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TIP60 antibody used in Western Blot (WB). GTX112197

GTX112197 WB Image

Jurkat whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TIP60 antibody (GTX112197) diluted at 1:1000.

Anti-TIP60 antibody used in Western Blot (WB). GTX112197

GTX112197 WB Image

TIP60 antibody detects KAT5 protein by western blot analysis.
A. 50 μg mouse brain lysate/extract
7.5% SDS-PAGE
TIP60 antibody (GTX112197) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169