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TIP60 antibody

Orthogonal
Anti-TIP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX112198
Anti-TIP60 antibody used in Western Blot (WB). GTX112198
Anti-TIP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX112198
Anti-TIP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX112198
Anti-TIP60 antibody used in Western Blot (WB). GTX112198

Cat. No. GTX112198

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all TIP60 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TIP60. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11165607

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lysine acetyltransferase 5 , ESA1 , HTATIP , HTATIP1 , PLIP , TIP , TIP60 , ZC2HC5 , cPLA2

Cellular Localization

Nucleus , nucleolus , Cytoplasm , perinuclear region

Background

The protein encoded by this gene belongs to the MYST family of histone acetyl transferases (HATs) and was originally isolated as an HIV-1 TAT-interactive protein. HATs play important roles in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins. This protein is a histone acetylase that has a role in DNA repair and apoptosis and is thought to play an important role in signal transduction. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TIP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX112198

GTX112198 IHC-P Image

TIP60 antibody detects TIP60 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
TIP60 stained by TIP60 antibody (GTX112198) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TIP60 antibody used in Western Blot (WB). GTX112198

GTX112198 WB Image

Orthogonal

Jurkat whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TIP60 antibody (GTX112198) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TIP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX112198

GTX112198 IHC-P Image

TIP60 antibody detects TIP60 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
TIP60 stained by TIP60 antibody (GTX112198) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TIP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX112198

GTX112198 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using TIP60(GTX112198) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TIP60 antibody used in Western Blot (WB). GTX112198

GTX112198 WB Image

The data was published in the journal Oncotarget in 2016. PMID: 26625199

REFERENCE

Application Reference

Chen Z et al. J Clin Invest 2021; 131 (19) Circular RNA cia-MAF drives self-renewal and metastasis of liver tumor-initiating cells via transcription factor MAFF.
Wright DG et al. Oncotarget 2016; 7 (2) : 1687-706 Human T-cell leukemia virus type-1-encoded protein HBZ represses p53 function by inhibiting the acetyltransferase activity of p300/CBP and HBO1.
Application : WB
Reactivity : Human
Collins HM et al. BMC Cancer 2013; 13 : 37 Differential effects of garcinol and curcumin on histone and p53 modifications in tumour cells.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169
Currently in production