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TIP60 antibody

Anti-TIP60 antibody used in Western Blot (WB). GTX112198
Anti-TIP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX112198
Anti-TIP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX112198
Anti-TIP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX112198

Cat No. GTX112198

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

Jurkat , Jurkat nucleus

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TIP60. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11165607

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

lysine acetyltransferase 5 , ESA1 , HTATIP , HTATIP1 , PLIP , TIP , TIP60 , ZC2HC5 , cPLA2

Cellular Localization

Nucleus , nucleolus , Cytoplasm , perinuclear region

Background

The protein encoded by this gene belongs to the MYST family of histone acetyl transferases (HATs) and was originally isolated as an HIV-1 TAT-interactive protein. HATs play important roles in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins. This protein is a histone acetylase that has a role in DNA repair and apoptosis and is thought to play an important role in signal transduction. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TIP60 antibody used in Western Blot (WB). GTX112198

GTX112198 WB Image

Jurkat whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TIP60 antibody (GTX112198) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TIP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX112198

GTX112198 IHC-P Image

TIP60 antibody detects TIP60 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
TIP60 stained by TIP60 antibody (GTX112198) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TIP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX112198

GTX112198 IHC-P Image

TIP60 antibody detects TIP60 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
TIP60 stained by TIP60 antibody (GTX112198) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TIP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX112198

GTX112198 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using TIP60(GTX112198) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

TIP60 antibody Cat No. GTX112198

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016. PMID: 26625199
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169