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TJP2 antibody

Anti-TJP2 antibody used in Western Blot (WB). GTX03355
Anti-TJP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX03355
Anti-TJP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX03355
Anti-TJP2 antibody used in Flow cytometry (FACS). GTX03355
Anti-TJP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03355
Anti-TJP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX03355

Cat. No. GTX03355

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μg ($429)
See all TJP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.25-0.5μg/ml
ICC/IF 5μg/ml
IHC-P 2-5μg/ml
FACS 1-3μg/1x10⁶ cells
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence of human TJP2/ZO2 (QEAQNARIEIAQKHPDIYAVPIK).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tight junction protein 2 , C9DUPq21.11 , DFNA51 , DUP9q21.11 , PFIC4 , X104 , ZO2

Cellular Localization

Cell membrane , Nucleus

Background

This gene encodes a zonula occluden that is a member of the membrane-associated guanylate kinase homolog family. The encoded protein functions as a component of the tight junction barrier in epithelial and endothelial cells and is necessary for proper assembly of tight junctions. Mutations in this gene have been identified in patients with hypercholanemia, and genomic duplication of a 270 kb region including this gene causes autosomal dominant deafness-51. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

Database

Research Area

DATA IMAGES

Anti-TJP2 antibody used in Western Blot (WB). GTX03355

GTX03355 WB Image

WB analysis of various sample lysates using GTX03355 TJP2 antibody.
Lane 1 : HEK293 whole cell lysates
Lane 2 : COS-7 whole cell lysates
Lane 3 : HeLa whole cell lysates
Lane 4 : MCF-7 whole cell lysates
Lane 5 : HepG2 whole cell lysates
Lane 6 : rat brain tissue lysates
Lane 7 : mouse brain tissue lysates
Lane 8 : HEPA1-6 whole cell lysates
Dilution : 0.5 μg/mL
Loading : 30μg per lane

Anti-TJP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX03355

GTX03355 IHC-P Image

IHC-P analysis of human rectal cancer tissue using GTX03355 TJP2 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution)
Dilution : 2μg/ml

Anti-TJP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX03355

GTX03355 IHC-P Image

IHC-P analysis of human ovarian serous adenocarcinoma tissue using GTX03355 TJP2 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution)
Dilution : 2μg/ml

Anti-TJP2 antibody used in Flow cytometry (FACS). GTX03355

GTX03355 FACS Image

FACS analysis of CACO-2 cells using GTX03355 TJP2 antibody.
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample
Antibody amount : 1μg/1x10⁶ cells for 30 min at 20ºC

Anti-TJP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03355

GTX03355 ICC/IF Image

ICC/IF analysis of A431 cells using GTX03355 TJP2 antibody. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins.
Green : Primary antibody
Blue : DAPI
Dilution : 5μg/mL

Anti-TJP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX03355

GTX03355 IHC-P Image

IHC-P analysis of human ovarian cancer tissue using GTX03355 TJP2 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution)
Dilution : 2μg/ml

REFERENCE

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REVIEW

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Package List Price ($)
$ 429