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TKTL1 antibody [N1C1]

Anti-TKTL1 antibody [N1C1] used in Western Blot (WB). GTX109459
Anti-TKTL1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX109459
Anti-TKTL1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109459

Cat No. GTX109459

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.98 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TKTL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038129

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

transketolase like 1 , TKR , TKT2

Database

Research Area

DATA IMAGES

Anti-TKTL1 antibody [N1C1] used in Western Blot (WB). GTX109459

GTX109459 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TKTL1 antibody [N1C1] (GTX109459) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TKTL1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX109459

GTX109459 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SAS xenograft , using Transketolase-like protein 1(GTX109459) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TKTL1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109459

GTX109459 ICC/IF Image

TKTL1 antibody [N1C1] detects TKTL1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TKTL1 protein stained by TKTL1 antibody [N1C1] (GTX109459) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar = 10 μm.

REFERENCE

REVIEW

TKTL1 antibody [N1C1] Cat No. GTX109459

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Exp Clin Cancer Res in 2019.PMID: 30971297
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Aging Cell in 2014.PMID: 24757718
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf