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TLE1 antibody

KO/KD
Anti-TLE1 antibody used in Western Blot (WB). GTX129993
Anti-TLE1 antibody used in Immunoprecipitation (IP). GTX129993
Anti-TLE1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX129993
Anti-TLE1 antibody used in Western Blot (WB). GTX129993

Cat. No. GTX129993

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Fr, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all TLE1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-Fr 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

293T , HepG2

Predict Reactivity

Rat, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.31 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human TLE1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886148

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TLE family member 1, transcriptional corepressor , ESG , ESG1 , GRG1

Cellular Localization

Nucleus

Background

Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.

Database

Research Area

DATA IMAGES

Anti-TLE1 antibody used in Western Blot (WB). GTX129993

GTX129993 WB Image

KO/KD

Wild-type (WT) and TLE1 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TLE1 antibody (GTX129993) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TLE1 antibody used in Immunoprecipitation (IP). GTX129993

GTX129993 IP Image

Immunoprecipitation of TLE1 protein from 293T whole cell extracts using 5 μg of TLE1 antibody (GTX129993).
Western blot analysis was performed using TLE1 antibody (GTX129993).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-TLE1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX129993

GTX129993 IHC-Fr Image

TLE1 antibody detects TLE1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebral cortex.
Green: TLE1 stained by TLE1 antibody (GTX129993) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-TLE1 antibody used in Western Blot (WB). GTX129993

GTX129993 WB Image

TLE1 antibody detects TLE1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TLE1 antibody (GTX129993) diluted at a dilution of 1:1000.

REFERENCE

Application Reference

Umur LF et al. Cureus 2021; 13 (2) : e13073 Intravenous Leiomyosarcoma of the Lower Extremity: As Peripheral as It Gets.
Reactivity : Human
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169