*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
NT2D1 , IMR32 , Jurkat , HeLa
Mouse, Bovine, Chicken, Xenopus laevis(>80% identity)
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.95 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human TLE4. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Transducin Like Enhancer Of Split 4,Bce-1,Bce1,E(Spi),Esg,Esg4,Grg4,Grg-4,Tle4
Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES.
GTX114842 ICC/IF Image
TLE4 antibody [C1C3] detects TLE4 protein by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TLE4 stained by TLE4 antibody [C1C3] (GTX114842) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
GTX114842 WB Image
Sample (30 ug of whole cell lysate)
7.5% SDS PAGE
GTX114842 diluted at 1:1000
GTX114842 IHC-P Image
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using TLE4(GTX114842) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min