GeneTex
United States (US)

TLE4 antibody [C1C3]

GTX114842_40233_20181227_ICC_IF_R_19022001_680.jpg
GTX114842_WB_18121410_584.jpg
GTX114842_40233_IHC_18121410_177.jpg

Cat No. GTX114842

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

10, 84 kDa. ( Note )

Positive Control

NT2D1 , IMR32 , Jurkat , HeLa

Predict Reactivity

Mouse, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TLE4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Transducin Like Enhancer Of Split 4,Bce-1,Bce1,E(Spi),Esg,Esg4,Grg4,Grg-4,Tle4

Background

Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES.

Research Area

DATA IMAGES
GTX114842_40233_20181227_ICC_IF_R_19022001_680.jpg

GTX114842 ICC/IF Image

TLE4 antibody [C1C3] detects TLE4 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TLE4 stained by TLE4 antibody [C1C3] (GTX114842) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX114842_WB_18121410_584.jpg

GTX114842 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
7.5% SDS PAGE
GTX114842 diluted at 1:1000

GTX114842_40233_IHC_18121410_177.jpg

GTX114842 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using TLE4(GTX114842) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169