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TLK1 antibody [N2C2], Internal

Overexpression
Anti-TLK1 antibody [N2C2], Internal used in Western Blot (WB). GTX102891
Anti-TLK1 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102891
Anti-TLK1 antibody [N2C2], Internal used in Western Blot (WB). GTX102891

Cat. No. GTX102891

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TLK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TLK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952265

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tousled like kinase 1 , PKU-beta

Cellular Localization

Nucleus

Background

The Tousled-like kinases, first described in Arabadopsis, are nuclear serine/threonine kinases that are potentially involved in the regulation of chromatin assembly.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-TLK1 antibody [N2C2], Internal used in Western Blot (WB). GTX102891

GTX102891 WB Image

Sample(30 μg of whole cell lysate)
A:293T
7.5% SDS PAGE
GTX102891 diluted at 1:500

Anti-TLK1 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102891

GTX102891 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TLK1(GTX102891) antibody at 1:200 dilution.

Anti-TLK1 antibody [N2C2], Internal used in Western Blot (WB). GTX102891

GTX102891 WB Image

Overexpression

The data was published in the journal BMC Mol Biol in 2016.PMID: 26860083

REFERENCE

Application Reference

Liaw GJ et al. Sci Rep 2019; 9 (1) : 1885 Inactive Tlk associating with Tak1 increases p38 MAPK activity to prolong the G2 phase.
Application : WB, IP
Singh V et al. Cell Cycle 2017; : 43112 Identification of the proteome complement of humanTLK1 reveals it binds and phosphorylates NEK1 regulating its activity.
Application : WB
Reactivity : Human
Awate S et al. BMC Mol Biol 2016; 17 : 3 TLK1B mediated phosphorylation of Rad9 regulates its nuclear/cytoplasmic localization and cell cycle checkpoint.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169