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TLK2 antibody

KO/KD
Anti-TLK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32923
Anti-TLK2 antibody used in Western Blot (WB). GTX32923
Anti-TLK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX32923
Anti-TLK2 antibody used in Western Blot (WB). GTX32923
Anti-TLK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX32923

Cat. No. GTX32923

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all TLK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human TLK2 (NP_006843.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887686

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tousled like kinase 2 , HsHPK , MRD57 , PKU-ALPHA

Cellular Localization

Nucleus,Cytoplasm, cytoskeleton

Background

This gene encodes a nuclear serine/threonine kinase that was first identified in Arabidopsis. The encoded protein is thought to function in the regulation of chromatin assembly in the S phase of the cell cycle by regulating the levels of a histone H3/H4 chaperone. This protein is associated with double-strand break repair of DNA damage caused by radiation. Pseudogenes of this gene are present on chromosomes 10 and 17. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Database

Research Area

DATA IMAGES

Anti-TLK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32923

GTX32923 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX32923 TLK2 antibody.
Blue : DAPI

Anti-TLK2 antibody used in Western Blot (WB). GTX32923

GTX32923 WB Image

WB analysis of various sample lysates using GTX32923 TLK2 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-TLK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX32923

GTX32923 IHC-P Image

IHC-P analysis of human esophageal cancer tissue using GTX32923 TLK2 antibody.
Dilution : 1:100

Anti-TLK2 antibody used in Western Blot (WB). GTX32923

GTX32923 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) NIH/3T3 cell lysate using GTX32923 TLK2 antibody.
Dilution : 1:500
Loading : 25μg per lane

Anti-TLK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX32923

GTX32923 IHC-P Image

IHC-P analysis of human liver damage tissue using GTX32923 TLK2 antibody.
Dilution : 1:100

REFERENCE

Application Reference

Huang TH et al. Mol Cell 2018; 69 (5) : 879-892.e5 The Histone Chaperones ASF1 and CAF-1 Promote MMS22L-TONSL-Mediated Rad51 Loading onto ssDNA during Homologous Recombination in Human Cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399