GeneTex
United States (US)

TLP antibody

GTX104694_39820_IHC_M_19011506_715.jpg
GTX104694_39820_IHC_19011506_868.jpg
GTX104694_39820_WB_M_brain_19011506_612.jpg
GTX104694_39820_IHC_M_2_19011506_319.jpg
GTX104694_39820_IFA_19011506_397.jpg
GTX104694_WB_19011506_466.jpg

Cat No. GTX104694

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

293T , mouse brain

Predict Reactivity

Rat, Bovine, Dog, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TLP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tata-Box Binding Protein Like 1,Mgc:8389,Mgc:9620,Stud,Tlf,Tlp,Trf2,Tbpl1

Cellular Localization

Cytoplasm , Nucleus

Background

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a protein that serves the same function as TBP and substitutes for TBP at some promoters that are not recognized by TFIID. It is essential for spermiogenesis and believed to be important in expression of developmentally regulated genes. [provided by RefSeq]

Database

DATA IMAGES
GTX104694_39820_IHC_M_19011506_715.jpg

GTX104694 IHC-P Image

TLP antibody detects TLP protein at cytosol on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
TLP antibody (GTX104694) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104694_39820_IHC_19011506_868.jpg

GTX104694 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using TLP(GTX104694) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104694_39820_WB_M_brain_19011506_612.jpg

GTX104694 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX104694 diluted at 1:500

GTX104694_39820_IHC_M_2_19011506_319.jpg

GTX104694 IHC-P Image

TLP antibody detects TLP protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
TLP antibody (GTX104694) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104694_39820_IFA_19011506_397.jpg

GTX104694 ICC/IF Image

TLP antibody detects TLP protein at cytoplasm by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TLP protein stained by TLP antibody (GTX104694) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX104694_WB_19011506_466.jpg

GTX104694 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
12% SDS PAGE
GTX104694 diluted at 1:2000

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169