GeneTex
United States (US)

TLR2 antibody [N1N2], N-term

GTX102577_40744_WB_B_19011506_451.jpg
GTX102577_40744_20141128_IHC_19011506_620.jpg

Cat No. GTX102577

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

90 kDa. ( Note )

Positive Control

Recombinant TLR2 protein

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human TLR2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CD282 antibody, TIL4 antibody, TLR2 antibody, toll/interleukin-1 receptor-like protein 4 antibody, toll/interleukin 1 receptor-like 4 antibody, toll-like receptor 2 antibody

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. [provided by RefSeq]
Show More +

Database

Research Area

DATA IMAGES
GTX102577_40744_WB_B_19011506_451.jpg

GTX102577 WB Image

Sample (30 ug of whole cell lysate)
A: Non-transfected 293T lysates
B: TLR2 transfected 293T lysates
7.5% SDS PAGE
GTX102577 diluted at 1:5000

GTX102577_40744_20141128_IHC_19011506_620.jpg

GTX102577 IHC-P Image

TLR2 antibody [N1N2], N-term detects TLR2 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
TLR2 antibody [N1N2], N-term (GTX102577) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf