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TLR3 antibody [HL2314]

Recombinant antibody Orthogonal
Anti-TLR3 antibody [HL2314] used in Western Blot (WB). GTX638477
Anti-TLR3 antibody [HL2314] used in Western Blot (WB). GTX638477
Anti-TLR3 antibody [HL2314] used in IHC (Paraffin sections) (IHC-P). GTX638477
Anti-TLR3 antibody [HL2314] used in IHC (Paraffin sections) (IHC-P). GTX638477
Anti-TLR3 antibody [HL2314] used in IHC (Paraffin sections) (IHC-P). GTX638477

Cat. No. GTX638477

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2314

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all TLR3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

104 kDa. ( Note )

Product Note

The immunogen sequence of this antibody is within cleaved form TLR3 protein, so this antibody is expected to detect both full-length and cleaved form TLR3 protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human TLR3.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

toll like receptor 3 , CD283 , IIAE2

Cellular Localization

Endosome membrane,Early endosome

Background

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. It may thus play a role in host defense against viruses. Use of alternative polyadenylation sites to generate different length transcripts has been noted for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TLR3 antibody [HL2314] used in Western Blot (WB). GTX638477

GTX638477 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TLR3 antibody [HL2314] (GTX638477) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TLR3 antibody [HL2314] used in Western Blot (WB). GTX638477

GTX638477 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TLR3 antibody [HL2314] (GTX638477) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-TLR3 antibody [HL2314] used in IHC (Paraffin sections) (IHC-P). GTX638477

GTX638477 IHC-P Image

TLR3 antibody [HL2314] detects TLR3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog brain.
TLR3 stained by TLR3 antibody [HL2314] (GTX638477) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TLR3 antibody [HL2314] used in IHC (Paraffin sections) (IHC-P). GTX638477

GTX638477 IHC-P Image

TLR3 antibody [HL2314] detects TLR3 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
TLR3 stained by TLR3 antibody [HL2314] (GTX638477) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TLR3 antibody [HL2314] used in IHC (Paraffin sections) (IHC-P). GTX638477

GTX638477 IHC-P Image

TLR3 antibody [HL2314] detects TLR3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
TLR3 stained by TLR3 antibody [HL2314] (GTX638477) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations