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TLR3 antibody

Comparison
Anti-TLR3 antibody used in Western Blot (WB). GTX113022
Anti-TLR3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113022
Anti-TLR3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113022
Anti-TLR3 antibody used in Western Blot (WB). GTX113022
Anti-TLR3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113022
Anti-TLR3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113022

Cat. No. GTX113022

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all TLR3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

104 kDa. ( Note )

Predict Reactivity

Rabbit, Sheep, Bovine, Cat, Guinea pig, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TLR3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952277

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

toll like receptor 3 , CD283 , IIAE2

Cellular Localization

Endoplasmic reticulum membrane; Single-pass type I membrane protein , Endosome membrane

Background

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. It may thus play a role in host defense against viruses. Use of alternative polyadenylation sites to generate different length transcripts has been noted for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TLR3 antibody used in Western Blot (WB). GTX113022

GTX113022 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with TLR3 antibody (GTX113022) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-TLR3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113022

GTX113022 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using TLR3(GTX113022) antibody at 1:200 dilution.

Anti-TLR3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113022

GTX113022 IHC-P Image

TLR3 antibody detects TLR3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
TLR3 stained by TLR3 antibody (GTX113022) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TLR3 antibody used in Western Blot (WB). GTX113022

GTX113022 WB Image

Comparison

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membranes were blotted with TLR3 antibody (GTX113022) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-TLR3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113022

GTX113022 IHC-P Image

Immunohistochemical analysis of paraffin-embedded TOV-112D xenograft, using TLR3(GTX113022) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TLR3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113022

GTX113022 IHC-P Image

TLR3 antibody detects TLR3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat pancreas.
TLR3 stained by TLR3 antibody (GTX113022) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Fukuyama K et al. Front Immunol 2023; 14 : 1117102 Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epithelium.
Bai Y et al. Mol Immunol 2021; 136 : 110-117 Poly (I: C) inhibits reticuloendothelial virus replication in chicken macrophage-like cells through the activation of toll-like receptor-3 signaling.
Application : WB
Song X et al. Oncotarget 2016; 7 (23) : 33796-808 HCV core protein binds to gC1qR to induce A20 expression and inhibit cytokine production through MAPKs and NF-κB signaling pathways.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169