TLR4 antibody [76B357.1]

Name: TLR4 antibody [76B357.1]
Brand: GeneTex
SKU: GTX57153
Price: 449.00 USD

Anti-TLR4 antibody [76B357.1] used in Western Blot (WB). GTX57153

Anti-TLR4 antibody [76B357.1] used in Flow cytometry (FACS). GTX57153

Anti-TLR4 antibody [76B357.1] used in IHC (Paraffin sections) (IHC-P). GTX57153

Cat. No. GTX57153

Reference 3

Host	Mouse
Clonality	Monoclonal
Clone Name	76B357.1
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS, ChIP assay, in vitro, MS
Reactivity	Human, Mouse, Rat, Bovine, Hamster, Pig, Baboon
Package	100 μg ($449)

See all TLR4 products

View product citations for antibody GTX57153 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1 - 3 μg/ml
ICC/IF	1:10 - 1:500
IHC-P	5 μg/ml
IHC-Fr	1:500
FACS	Assay dependent
ChIP assay	1:10 - 1:500
in vitro	Assay dependent
MS	Assay dependent

Not tested in other applications.

Calculated MW

96 kDa. ( Note )

Predict Reactivity

Sheep(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This antibody was developed against a portion of amino acids 100-200 of human TLR4 (NP_612564).

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_2887949

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

toll like receptor 4 , ARMD10 , CD284 , TLR-4 , TOLL

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. It has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Also, several transcript variants of this gene have been found, but the protein coding potential of most of them is uncertain. [provided by RefSeq]

Database

Gene ID: 7099 TLR4
UniProt: O00206 TLR4

Research Area

DATA IMAGES

GTX57153 WB Image

WB analysis of (A) human (B) mouse, and (C) rat intestine tissue lysate using GTX57153 TLR4 antibody [76B357.1].
Dilution : 2 μg/ml(A) ; 6μg/ml(B and C)

GTX57153 FACS Image

FACS analysis of human monocyte-derived dendritic cells using GTX57153 TLR4 antibody [76B357.1].
Blue : Primary antibody
Green : negative control
Black : unstained cells
Dilution : 1:200

GTX57153 IHC-P Image

IHC-P analysis of rat salivary gland tissue using GTX57153 TLR4 antibody [76B357.1].
Dilution : 1:100

GTX57153 IHC-P Image

IHC-P analysis of human small intestine malignant stromal tumor tissue using GTX57153 TLR4 antibody [76B357.1].
Dilution : 5 μg/ml

GTX57153 FACS Image

FACS analysis of THP-1 cells using GTX57153 TLR4 antibody [76B357.1].
Blue : Isotype control
Green : Primary antibody
Antibody amount : 2 μg10^6 cells

GTX57153 FACS Image

FACS (Intracellular staining) analysis of Jurkat cells using GTX57153 TLR4 antibody [76B357.1].
Blue : Primary antibody
Orange : isotype control
Dilution : 2.5 μg/ml
Fixation : 4% PFA
Permibilization : 0.1% saponin

GTX57153 IHC-P Image

IHC-P analysis of human skin tissue using GTX57153 TLR4 antibody [76B357.1].
Dilution : 5 μg/ml

GTX57153 IHC-P Image

IHC-P analysis of human colon tissue using GTX57153 TLR4 antibody [76B357.1].
TOP : isotype control
Bottom : primary antibody
Dilution : 5 μg/ml

GTX57153 IHC-P Image

IHC-P analysis of human breast tissue using GTX57153 TLR4 antibody [76B357.1].
Dilution : 5 μg/ml

GTX57153 FACS Image

FACS analysis of human peripheral blood mononuclear cell (PBMC) using GTX57153 TLR4 antibody [76B357.1]. Cells were co-stained with anti-CD14 antibody. CD14+ cells were gated for analysis.
Left : Isotype control
Right : Primary antibody
Antibody amount : 1μg

GTX57153 FACS Image

FACS analysis of mouse splenocytes using GTX57153 TLR4 antibody [76B357.1].
Red : Primary antibody
Green : isotype control
Shaded histogram : cell only
Antibody amount : 0.5 μg/10^6 cells

GTX57153 IHC-P Image

IHC-P analysis of human esophageal squamous cell carcinoma (SCC) tissue using GTX57153 TLR4 antibody [76B357.1].
Dilution : 5 μg/ml

GTX57153 IHC-P Image

IHC-P analysis of human skin tissue using GTX57153 TLR4 antibody [76B357.1].
Dilution : 5 μg/ml
Antigen retrieval : 10 mM sodium citrate buffer, pH 6.0

GTX57153 IHC-P Image

IHC-P analysis of human testis tissue using GTX57153 TLR4 antibody [76B357.1].
Dilution : 5 μg/ml

REFERENCE

Application Reference

H Cui et al. Research Square 2023; TNF-α promotes osteocyte necroptosis by upregulating TLR4 in postmenopausal osteoporosis

Application : WB

Liu X et al. Mol Pain 2023; 19 : 1.74481E+16 Toll-like receptor 4 signaling pathway in sensory neurons mediates remifentanil-induced postoperative hyperalgesia via transient receptor potential ankyrin 1.

Application : WB, IHC-Fr

Reactivity : Rat

Karpova T et al. Life Sci 2020; 245 : 117365 Blockade of Toll-like receptor 4 (TLR4) reduces oxidative stress and restores phospho-ERK1/2 levels in Leydig cells exposed to high glucose.

Application : IHC-P

Reactivity : Human

Submit a Reference

REVIEW

There are currently no reviews for TLR4 antibody [76B357.1] (GTX57153). Be the first to share your experience with this product.

Submit a Review

Application Reference

H Cui et al. Research Square 2023; TNF-α promotes osteocyte necroptosis by upregulating TLR4 in postmenopausal osteoporosis

Application : WB

Liu X et al. Mol Pain 2023; 19:1.74481E+16 Toll-like receptor 4 signaling pathway in sensory neurons mediates remifentanil-induced postoperative hyperalgesia via transient receptor potential ankyrin 1.

Application : WB, IHC-Fr

Reactivity : Rat

Karpova T et al. Life Sci 2020; 245:117365 Blockade of Toll-like receptor 4 (TLR4) reduces oxidative stress and restores phospho-ERK1/2 levels in Leydig cells exposed to high glucose.

Application : IHC-P

Reactivity : Human

SDS
PBS.pdf
Sodium Azide.pdf

Package	List Price ($)
100 μg	$ 449
Qty

TLR4 antibody [76B357.1]

Cat. No. GTX57153

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Predict Reactivity

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX57153 WB Image

GTX57153 FACS Image

GTX57153 IHC-P Image

GTX57153 IHC-P Image

GTX57153 FACS Image

GTX57153 FACS Image

GTX57153 IHC-P Image

GTX57153 IHC-P Image

GTX57153 IHC-P Image

GTX57153 FACS Image

GTX57153 FACS Image

GTX57153 IHC-P Image

GTX57153 IHC-P Image

GTX57153 IHC-P Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

© 1998-2022 GeneTex, Inc. All rights reserved.

All products are for research use only—Not for use in diagnostic or therapeutic applications.