GeneTex
United States (US)

TLR4 antibody [HTA125] (FITC)

GTX28378_5590_FACS_18121410_964.jpg

Cat No. GTX28378

Host Mouse
Clonality Monoclonal
Clone Name HTA125
Isotype IgG2a
Application ICC/IF, FACS
Reactivity Human, Dog, Guinea pig, Pig, Rhesus Monkey
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF Assay dependent
FACS Neat

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood.

Not tested in other applications.

Calculated MW

96 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide, 1% BSA

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Ba/F3 cell line expressing TLR4 (CD284).

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Fluorescein isothiocyanate (FITC)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Toll Like Receptor 4,Armd10,Cd284,Tlr-4,Toll,Tlr4

Background

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Database

Research Area

DATA IMAGES
GTX28378_5590_FACS_18121410_964.jpg

GTX28378 FACS Image

FACS analysis of human peripheral blood monocytes using GTX28378 TLR4 antibody [HTA125] (FITC).

REFERENCE