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TLR4 antibody [HTA125]

Orthogonal
Anti-TLR4 antibody [HTA125] used in Flow cytometry (FACS). GTX75742
Anti-TLR4 antibody [HTA125] used in Western Blot (WB). GTX75742

Cat. No. GTX75742

Reference 31

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 HTA125

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, Blocking

Reactivity

 Human, Mouse, Cat, Dog, Guinea pig, Pig, Rhesus Monkey
Package
100 μg ($389)
See all TLR4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS 1/10-1/25
IP Assay dependent
Blocking Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood.
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Ba/F3 cell line expressing TLR4 (CD284).

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_377004

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

toll like receptor 4 , ARMD10 , CD284 , TLR-4 , TOLL

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Database

Research Area

DATA IMAGES

Anti-TLR4 antibody [HTA125] used in Flow cytometry (FACS). GTX75742

GTX75742 FACS Image

FACS analysis of human peripheral blood monocytes using GTX75742 TLR4 antibody [HTA125].

Anti-TLR4 antibody [HTA125] used in Western Blot (WB). GTX75742

GTX75742 WB Image

Orthogonal

The data was published in the 2022 in Front Pharmacol. PMID: 35308231

REFERENCE

Application Reference

Xue Zhang et al. Front Pharmacol 2022; 13 : 827128 A Polysaccharide From Eupolyphaga sinensis Walker With Anti-HBV Activities In Vitro and In Vivo.
Application : WB
Reactivity : Human
Kogan E et al. Diagn Pathol 2022; 17 (1) : 31 Morphologically, immunohistochemically and PCR proven lymphocytic viral peri-, endo-, myocarditis in patients with fatal COVID-19.
Application : IHC-P
Reactivity : Human
Chen LL et al. Evid Based Complement Alternat Med 2021; 2021 : 5205759 Suppression of Inflammatory and Fibrotic Signals by Cinnamon (Cinnamomum cassia) and Cinnamaldehyde in Cyclophosphamide-Induced Overactive Bladder in Mice.
Application : IHC-Fr
Reactivity : Mouse
Chien LH et al. Antioxidants (Basel) 2021; 10 (10)
Reactivity : Mouse
Weng J et al. Theranostics 2021; 11 (16) : 7620-7639 Fiber scaffold bioartificial liver therapy relieves acute liver failure and extrahepatic organ injury in pigs.
Application : IHC-P
Reactivity : Human
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REVIEW

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Application Reference
Xue Zhang et al. Front Pharmacol 2022; 13:827128 A Polysaccharide From Eupolyphaga sinensis Walker With Anti-HBV Activities In Vitro and In Vivo.
Application : WB
Reactivity : Human
Kogan E et al. Diagn Pathol 2022; 17 (1):31 Morphologically, immunohistochemically and PCR proven lymphocytic viral peri-, endo-, myocarditis in patients with fatal COVID-19.
Application : IHC-P
Reactivity : Human
Chen LL et al. Evid Based Complement Alternat Med 2021; 2021:5205759 Suppression of Inflammatory and Fibrotic Signals by Cinnamon (Cinnamomum cassia) and Cinnamaldehyde in Cyclophosphamide-Induced Overactive Bladder in Mice.
Application : IHC-Fr
Reactivity : Mouse
Chien LH et al. Antioxidants (Basel) 2021; 10 (10)
Reactivity : Mouse
Weng J et al. Theranostics 2021; 11 (16):7620-7639 Fiber scaffold bioartificial liver therapy relieves acute liver failure and extrahepatic organ injury in pigs.
Application : IHC-P
Reactivity : Human
Zhang S et al. Europe PMC 2021; (Epub) Resatorvid Modulates Microglial M1/M2 Polarization to Improve Cognitive Impairment after Repetitive Mild Traumatic Brain Injury.
Application : ICC/IF
Liu S et al. Front Microbiol 2020; 11:845 PE_PGRS31-S100A9 Interaction Promotes Mycobacterial Survival in Macrophages Through the Regulation of NF-κB-TNF-α Signaling and Arachidonic Acid Metabolism.
Huang CY et al. Nutrients 2020; 12 (6) Attenuation of Lipopolysaccharide-Induced Acute Lung Injury by Hispolon in Mice, Through Regulating the TLR4/PI3K/Akt/mTOR and Keap1/Nrf2/HO-1 Pathways, and Suppressing Oxidative Stress-Mediated ER Stress-Induced Apoptosis and Autophagy.
Reactivity : Mouse
Liu C et al. Inflammation 2019; 6-Bromoindirubin-3'-Oxime Suppresses LPS-Induced Inflammation via Inhibition of the TLR4/NF-κB and TLR4/MAPK Signaling Pathways.
Pei-Ying Li et al. RSC Adv. 2018; 8:31515-31528 Alpinumisoflavone attenuates lipopolysaccharide-induced acute lung injury by regulating the effects of anti-oxidation and anti-inflammation both in vitro and.
Reactivity : Mouse
Cao SG et al. Microb Pathog 2018; 116:313-317 Cryptotanshinone inhibits prostaglandin E2 production and COX-2 expression via suppression of TLR4/NF-κB signaling pathway in LPS-stimulated Caco-2 cells.
Reactivity : Human
Chao W et al. Int Immunopharmacol 2017; 50:77-86 3, 4-dihydroxybenzalacetone attenuates lipopolysaccharide-induced inflammation in acute lung injury via down-regulation of MMP-2 and MMP-9 activities through suppressing ROS-mediated MAPK and PI3K/AKT signaling pathways.
Reactivity : Mouse
Lin WC et al. RSC Adv. 2017; 7:21234-21251 Anti-inflammatory activity of Sanghuangporus sanghuang by suppressing the TLR4-mediated PI3K/AKT/mTOR/IKKβ signaling pathway.
Reactivity : Mouse
Zhang Z et al. Int Immunopharmacol 2016; (Epub) Zanthoxylum bungeanum pericarp extract prevents dextran sulfate sodium-induced experimental colitis in mice via the regulation of TLR4 and TLR4-related signaling pathways.
Reactivity : Mouse
Liu YH et al. Mar Drugs 2015; 13 (2):1026-36 Omega-3 fatty acid intervention suppresses lipopolysaccharide-induced inflammation and weight loss in mice.
Reactivity : Mouse
Chen WL et al. PLoS One 2015; 10 (9):e0137979 Mycobacterium tuberculosis Upregulates TNF-α Expression via TLR2/ERK Signaling and Induces MMP-1 and MMP-9 Production in Human Pleural Mesothelial Cells.
Application : FACS
Reactivity : Human
Previtera ML et al. PLoS One 2015; 10 (12):e0145813 Substrate Stiffness Regulates Proinflammatory Mediator Production through TLR4 Activity in Macrophages.
Reactivity : Mouse
Wei D et al. Inflammation 2014; 37 (4):1307-16 Anti-inflammatory effects of triptolide in LPS-induced acute lung injury in mice.
Reactivity : Mouse
Fu Y et al. J Ethnopharmacol 2013; 145 (1):193-9 Magnolol inhibits lipopolysaccharide-induced inflammatory response by interfering with TLR4 mediated NF-κB and MAPKs signaling pathways.
Reactivity : Human, Mouse
Hu K et al. Eur J Pharmacol 2013; 721 (43103):96-102 Alpinetin inhibits LPS-induced inflammatory mediator response by activating PPAR-γ in THP-1-derived macrophages.
Reactivity : Human
Krupa A et al. Am J Respir Cell Mol Biol 2013; 48 (2):240-9 Bruton's tyrosine kinase mediates FcγRIIa/Toll-like receptor-4 receptor crosstalk in human neutrophils.
Application : ICC/IF
Reactivity : Human
Tzeng HP et al. Am J Physiol Heart Circ Physiol 2008; 294 (3):H1490-6 Negative inotropic effects of high-mobility group box 1 protein in isolated contracting cardiac myocytes.
Application : Blocking
Reactivity : Cat
Henning LN et al. J Immunol 2008; 180 (12):7847-58 Pulmonary surfactant protein A regulates TLR expression and activity in human macrophages.
Application : ICC/IF
Reactivity : Human
Clone Reference
Li F et al. J Surg Res 2014; (Epub) Inhibitory effects of Astragalin on LPS-Induced Inflammatory Response in Mouse Mammary Epithelial Cells.
He Z et al. Exp Ther Med 2014; 8 (3):871-876 Toll-like receptor 4 monoclonal antibody attenuates lipopolysaccharide-induced acute lung injury in mice.
Liu J et al. Arch Anim Nutr 2014; 68 (2):111-25 Effects of intrauterine growth retardation and postnatal high-fat diet on hepatic inflammatory response in pigs.
Feng X et al. Inflammation 2014; Protective Effect of Carvacrol on Acute Lung Injury Induced by Lipopolysaccharide in Mice.
Tang Y et al. Eur J Pharmacol 2014; 723:494-500 Protective effect of cryptotanshinone on lipopolysaccharide-induced acute lung injury in mice.
Uehara A et al. J Med Microbiol 2002; 51 (8):626-34 priming of human oral epithelial cells by *-gamma to secrete cytokines in response to lipopolysaccharides, lipoteichoic acids and peptidoglycans.
Flo TH et al. J Biol Chem 2002; 277 (38):35489-95 Involvement of toll-like receptor (TLR) 2 and TLR4 in cell activation by mannuronic acid polymers.
Tabeta K et al. Infect Immun 2000; 68 (6):668881 Toll-like receptors confer responsiveness to lipopolysaccharide from Porphyromonas gingivalis in human gingival fibroblasts.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 389