GeneTex
United States (US)

TLR4 antibody [MTS510]

GTX29104_26_FACS_18121410_790.jpg
GTX29104_27_FACS_18121410_273.jpg

Cat No. GTX29104

Host Rat
Clonality Monoclonal
Clone Name MTS510
Isotype IgG2a
Application FACS, IP, Neut
Reactivity Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
FACS 1 - 2 μg / 10⁶ cells
IP 1:10 - 1:500
Neut Assay dependent
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

Specificity/Sensitivity

This antibody preferentially recognizes TLR4-MD-2 complex than of TLR4 alone.
PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

This antibody was developed by immunizing rats with the Ba/F3 cell line expressing mouse TLR4 and MD-2 (Akashi et al, 2000).

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Toll-Like Receptor 4,Lps,Ly87,Ran/M1,Rasl2-8,Tlr4

Cellular Localization

Membrane, Single-pass type I membrane protein

Background

Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.

Database

Research Area

DATA IMAGES
GTX29104_26_FACS_18121410_790.jpg

GTX29104 FACS Image

FACS analysis of mouse peritoneal cells using GTX29104 TLR4 antibody [MTS510].
Red : primary antibody
Green : isotype control

GTX29104_27_FACS_18121410_273.jpg

GTX29104 FACS Image

FACS analysis of RAW264.7 cells using GTX29104 TLR4 antibody [MTS510].
Red : Primary antibody
Green : isotype control
Black : cells without antibody
Antibody amount : 0.5μg

REFERENCE