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TLR4 antibody [MTS510]

Anti-TLR4 antibody [MTS510] used in Flow cytometry (FACS). GTX29104
Anti-TLR4 antibody [MTS510] used in Flow cytometry (FACS). GTX29104

Cat. No. GTX29104

Reference 1

Host

 Rat

Clonality

 Monoclonal

Clone Name

 MTS510

Isotype

 IgG2a

Application

 FACS, IP, Neutralizing/Inhibition

Reactivity

 Mouse
Package
100 μg ($399)
See all TLR4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS 1 - 2 μg / 10⁶ cells
IP 1:10 - 1:500
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

Product Note

This antibody preferentially recognizes TLR4-MD-2 complex than of TLR4 alone.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

This antibody was developed by immunizing rats with the Ba/F3 cell line expressing mouse TLR4 and MD-2 (Akashi et al, 2000).

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_371685

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

toll-like receptor 4 , Lps , Ly87 , Ran/M1 , Rasl2-8

Cellular Localization

Cell membrane

Background

Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.

Database

Research Area

DATA IMAGES

Anti-TLR4 antibody [MTS510] used in Flow cytometry (FACS). GTX29104

GTX29104 FACS Image

FACS analysis of RAW264.7 cells using GTX29104 TLR4 antibody [MTS510].
Red : Primary antibody
Green : isotype control
Black : cells without antibody
Antibody amount : 0.5μg

Anti-TLR4 antibody [MTS510] used in Flow cytometry (FACS). GTX29104

GTX29104 FACS Image

FACS analysis of mouse peritoneal cells using GTX29104 TLR4 antibody [MTS510].
Red : primary antibody
Green : isotype control

REFERENCE

Application Reference

Zhang W et al. J Am Heart Assoc 2015; 4 (6) : e001993 Necrotic myocardial cells release damage-associated molecular patterns that provoke fibroblast activation in vitro and trigger myocardial inflammation and fibrosis in vivo.
Application : Neutralizing/Inhibition
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399