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TLR4 antibody

Anti-TLR4 antibody used in Western Blot (WB). GTX64330
Anti-TLR4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64330

Cat. No. GTX64330

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, Multiplexing

Reactivity

 Human, Rat
Package
100 μl ($399)
See all TLR4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Multiplexing Assay dependent
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 500-600 of human TLR4 (NP_612564.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

toll like receptor 4 , ARMD10 , CD284 , TLR-4 , TOLL

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Database

Research Area

DATA IMAGES

Anti-TLR4 antibody used in Western Blot (WB). GTX64330

GTX64330 WB Image

WB analysis of HeLa cell lysates using GTX64330 TLR4 antibody.
Dilution : 1:2000
Loading : 25μg

Anti-TLR4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64330

GTX64330 ICC/IF Image

ICC/IF analysis of RAW264.7 cells using GTX64330 TLR4 antibody.
Orange : Primary antibody
Blue : DAPI
Dilution : 1:200

REFERENCE

Application Reference

Kang Wang et al. J Cell Mol Med 2022; 26 (10) : 2959-2971 Effect of TLR4/MyD88/NF-kB axis in paraventricular nucleus on ventricular arrhythmias induced by sympathetic hyperexcitation in post-myocardial infarction rats.
Application : WB, Multiplexing
Reactivity : Rat
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399