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TLR5 antibody

Anti-TLR5 antibody used in Western Blot (WB). GTX31724
Anti-TLR5 antibody used in IHC (Paraffin sections) (IHC-P). GTX31724

Cat. No. GTX31724

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all TLR5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 2 μg/mL
IHC-P 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

98 kDa. ( Note )

Positive Control

Rat Brain Tissue Lysate(GTX27942)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

TLR5 antibody was raised against a peptide corresponding to 16 amino acids near the center of human TLR5.The immunogen is located within amino acids 450 - 500 of TLR5.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

toll like receptor 5 , MELIOS , SLE1 , SLEB1 , TIL3

Cellular Localization

Cell membrane

Background

This gene encodes a member of the toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immune responses. These receptors recognize distinct pathogen-associated molecular patterns that are expressed on infectious agents. The protein encoded by this gene recognizes bacterial flagellin, the principal component of bacterial flagella and a virulence factor. The activation of this receptor mobilizes the nuclear factor NF-kappaB, which in turn activates a host of inflammatory-related target genes. Mutations in this gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.[provided by RefSeq, Dec 2009]

Database

Research Area

DATA IMAGES

Anti-TLR5 antibody used in Western Blot (WB). GTX31724

GTX31724 WB Image

WB analysis of rat brain tissue lysate using GTX31724 TLR5 antibody.
Working concentration : (A) 0.5, (B) 1, and (C) 2 μg/ml

Anti-TLR5 antibody used in IHC (Paraffin sections) (IHC-P). GTX31724

GTX31724 IHC-P Image

IHC-P analysis of rat brain tissue using GTX31724 TLR5 antibody.
Working concentration : 10 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399