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TLR7 antibody

Anti-TLR7 antibody used in IHC (Paraffin sections) (IHC-P). GTX125910
Anti-TLR7 antibody used in Western Blot (WB). GTX125910

Cat No. GTX125910

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($329),
25 μl ($169)
See all TLR7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

121 kDa. ( Note )

Positive Control

293T transfected recombinant TLR7 protein

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Cat, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human TLR7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

toll like receptor 7 , TLR7-like

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8, on chromosome X. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TLR7 antibody used in IHC (Paraffin sections) (IHC-P). GTX125910

GTX125910 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using TLR7 antibody(GTX125910) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TLR7 antibody used in Western Blot (WB). GTX125910

GTX125910 WB Image

Sample (30 ug of whole cell lysate)
A: Non-transfected 293T lysates
B: TLR7 transfected 293T lysates
5% SDS PAGE
GTX125910 diluted at 1:5000

REFERENCE

Application Reference

Wieczfinska J et al. Respir Res 2015; 16 (1) : 147 The whole-genome expression analysis of peripheral blood mononuclear cells from aspirin sensitive asthmatics versus aspirin tolerant patients and healthy donors after in vitro aspirin challenge.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169