APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 - 3 μg/ml |
| 1:5 - 1:1000 |
| Assay dependent |
| 1:5 - 1:1000 |
| 2 - 5 μg / 10⁶ cells |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 706-749 of human TLR7; GenBank no. gb|AAF78035.1|AF245702_1. It will cross-react with mouse TLR7. In human Ramos cells, additional bands are seen.
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
toll like receptor 7 , TLR7-like
Cellular Localization
Endoplasmic reticulum membrane,Endosome,Lysosome
Background
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8, on chromosome X. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX13730 FACS Image
FACS (Intracellular staining) analysis of human peripheral blood mononuclear cells (PBMCs) using GTX13730 TLR7 antibody.
Red : primary antibody
Green : isotype control
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GTX13730 ICC/IF Image
ICC/IF analysis of RAW264.7 cells using GTX13730 TLR7 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:10
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GTX13730 WB Image
WB analysis of RAW264.7 cell lysate using GTX13730 TLR7 antibody.
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GTX13730 FACS Image
FACS analysis of mouse splenocytes using GTX13730 TLR7 antibody.
Red : primary antibody
Green : isotype control
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GTX13730 ICC/IF Image
ICC/IF analysis of Ramos cells using GTX13730 TLR7 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:50
Fixation : 10% formalin
Permibilization : 0.5% Triton-X100
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GTX13730 FACS Image
FACS analysis of human peripheral blood mononuclear cell (PBMC) using GTX13730 TLR7 antibody.
Red : Primary antibody
Green : isotype control
Shaded histogram : cell only
Dilution : 0.5 μg/ml
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GTX13730 IHC-P Image
IHC-P analysis of human colon tissue using GTX13730 TLR7 antibody.
TOP : primary antibody
Bottom : isotype control
Dilution : 5 μg/ml
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GTX13730 FACS Image
FACS (Intracellular staining) analysis of human monocyte using GTX13730 TLR7 antibody.
Red : Primary antibody
Green : isotype control
Shaded histogram : cell only
Dilution : 0.5 μg/10⁶ cells
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REFERENCE
There are currently no references for TLR7 antibody (GTX13730). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for TLR7 antibody (GTX13730). Be the first to share your experience with this product.
