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TLR8 antibody [44C143]

Anti-TLR8 antibody [44C143] used in Flow cytometry (FACS). GTX12120
Anti-TLR8 antibody [44C143] used in Western Blot (WB). GTX12120
Anti-TLR8 antibody [44C143] used in Flow cytometry (FACS). GTX12120
Anti-TLR8 antibody [44C143] used in Western Blot (WB). GTX12120
Anti-TLR8 antibody [44C143] used in Flow cytometry (FACS). GTX12120
Anti-TLR8 antibody [44C143] used in Flow cytometry (FACS). GTX12120
Anti-TLR8 antibody [44C143] used in Flow cytometry (FACS). GTX12120
Anti-TLR8 antibody [44C143] used in IHC (Paraffin sections) (IHC-P). GTX12120

Cat. No. GTX12120

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 44C143

Isotype

 IgG1

Application

 WB, IHC-P, FACS, MS

Reactivity

 Human, Mouse
Package
50 μg ($399)
See all TLR8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 3 μg/ml
IHC-P 5 μg/ml
FACS 0.5 - 1 μg / 10⁶ cells
MS Assay dependent
Not tested in other applications.

Calculated MW

120 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This antibody was developed against a KLH-conjugated synthetic peptide of human TLR8, within amino acids 750-850.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

toll like receptor 8 , CD288

Cellular Localization

Membrane

Background

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung and peripheral blood leukocytes, and lies in close proximity to another family member, TLR7, on chromosome X. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TLR8 antibody [44C143] used in Flow cytometry (FACS). GTX12120

GTX12120 FACS Image

FACS (Intracellular staining) analysis of human lymphocytes using GTX12120 TLR8 antibody [44C143].
Red : Primary antibody
Green : isotype control
Shaded histogram : cell only

Anti-TLR8 antibody [44C143] used in Western Blot (WB). GTX12120

GTX12120 WB Image

WB analysis of Ramos (left) and human spleen (right) lysate using GTX12120 TLR8 antibody [44C143].

Anti-TLR8 antibody [44C143] used in Flow cytometry (FACS). GTX12120

GTX12120 FACS Image

FACS analysis of 10⁶ human lymphocytes using GTX12120 TLR8 antibody [44C143].
Red : Primary antibody
Green : isotype control
Shaded histogram : cell only
Antibody amount : 1 μg

Anti-TLR8 antibody [44C143] used in Western Blot (WB). GTX12120

GTX12120 WB Image

WB analysis of Ramos cell lysate using GTX12120 TLR8 antibody [44C143].

Anti-TLR8 antibody [44C143] used in Flow cytometry (FACS). GTX12120

GTX12120 FACS Image

FACS (Intracellular staining) analysis of Ramos cells using GTX12120 TLR8 antibody [44C143].
Red : Primary antibody
Green : isotype control
Shaded histogram : cell only
Antibody amount : 2 μg

Anti-TLR8 antibody [44C143] used in Flow cytometry (FACS). GTX12120

GTX12120 FACS Image

FACS analysis of mouse splenocytes using GTX12120 TLR8 antibody [44C143].
Red : primary antibody
Green : isotype control
Antibody amount : 2μg

Anti-TLR8 antibody [44C143] used in Flow cytometry (FACS). GTX12120

GTX12120 FACS Image

FACS (Intracellular staining) analysis of (A) A549 cells (B) 293T cells(as negative control) using GTX12120 TLR8 antibody [44C143].
Blue : Primary antibody
Orange : isotype control
Dilution : 5 μg/ml
Fixation : 4% PFA
Permibilization : 0.1% saponin

Anti-TLR8 antibody [44C143] used in IHC (Paraffin sections) (IHC-P). GTX12120

GTX12120 IHC-P Image

IHC-P analysis of human spleen tissue using GTX12120 TLR8 antibody [44C143].
Dilution : 2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399