TLR9 antibody [N1N2], N-term

Name: TLR9 antibody [N1N2], N-term
Brand: GeneTex
SKU: GTX111547
Price: 399.00 USD
Rating: 5 (2 reviews)

Anti-TLR9 antibody [N1N2], N-term used in Western Blot (WB). GTX111547

Anti-TLR9 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111547

Anti-TLR9 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX111547

Cat. No. GTX111547

Reference 3

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Tupaia chinensis
Package	100 μl ($399), 25 μl ($169)

See all TLR9 products

View product citations for antibody GTX111547 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

116 kDa. ( Note )

Positive Control

TLR9 transfected 293T

Predict Reactivity

Sheep, Bovine, Cat, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human TLR9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952286

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

toll like receptor 9 , CD289

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response. [provided by RefSeq]

Database

Gene ID: 54106 TLR9
UniProt: Q9NR96 TLR9

Research Area

DATA IMAGES

GTX111547 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TLR9 antibody [N1N2], N-term (GTX111547) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

GTX111547 ICC/IF Image

TLR9 antibody [N1N2], N-term detects TLR9 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TLR9 stained by TLR9 antibody [N1N2], N-term (GTX111547) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX111547 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TLR9 antibody [N1N2], N-term (GTX111547) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX111547 ICC/IF Image

TLR9 antibody [N1N2], N-term detects TLR9 protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min.
Green: TLR9 protein stained by TLR9 antibody [N1N2], N-term (GTX111547) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX111547 IHC-P Image

TLR9 antibody [N1N2], N-term detects TLR9 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
TLR9 stained by TLR9 antibody [N1N2], N-term (GTX111547) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX111547 WB Image

Sample (30 μg of whole cell lysate)
A: Non-transfected 293T lysates
B: TLR9 transfected 293T lysates
7.5% SDS PAGE
GTX111547 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX111547 IHC-P Image

TLR9 antibody [N1N2], N-term detects TLR9 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse placenta.
TLR9 stained by TLR9 antibody [N1N2], N-term (GTX111547) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Kogan E et al. Diagn Pathol 2022; 17 (1) : 31 Morphologically, immunohistochemically and PCR proven lymphocytic viral peri-, endo-, myocarditis in patients with fatal COVID-19.

Application : IHC-P

Reactivity : Human

Yanagawa M et al. J Gastroenterol 2017; (Epub) Basophils activated via TLR signaling may contribute to pathophysiology of type 1 autoimmune pancreatitis.

Application : IHC-P

Reactivity : Human

O'Hara JR et al. Infect Immun 2012; 80 (4) : 1563-71 Campylobacter jejuni disrupts protective Toll-like receptor 9 signaling in colonic epithelial cells and increases the severity of dextran sulfate sodium-induced colitis in mice.

Application : WB

Reactivity : Mouse

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