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TLR9 antibody

Anti-TLR9 antibody used in IHC (Paraffin sections) (IHC-P). GTX31294
Anti-TLR9 antibody used in Western Blot (WB). GTX31294
Anti-TLR9 antibody used in IHC (Paraffin sections) (IHC-P). GTX31294

Cat. No. GTX31294

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all TLR9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 1 μg/mL
IHC-P 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

116 kDa. ( Note )

Positive Control

Mouse Spleen Tissue Lysate(GTX27937)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

TLR9 antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human TLR9.The immunogen is located within amino acids 960 - 1010 of TLR9.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

toll like receptor 9 , CD289

Cellular Localization

Endoplasmic reticulum membrane,Endosome,Lysosome

Background

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TLR9 antibody used in IHC (Paraffin sections) (IHC-P). GTX31294

GTX31294 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX31294 TLR9 antibody.
Working concentration : 2 μg/ml

Anti-TLR9 antibody used in Western Blot (WB). GTX31294

GTX31294 WB Image

WB analysis of mouse spleen tissue lysate using GTX31294 TLR9 antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml

Anti-TLR9 antibody used in IHC (Paraffin sections) (IHC-P). GTX31294

GTX31294 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX31294 TLR9 antibody.
Working concentration : 10 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399