GeneTex
United States (US)

TLR9 antibody

GTX31295_894_IHC-P_20180221_18121410_163.jpg
GTX31295_2498_WB_20180221_18121410_577.jpg
GTX31295_104_ICC-IF_20180221_18121410_678.jpg

Cat No. GTX31295

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/mL
ICC/IF 2 μg/mL
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

116 kDa. ( Note )

Positive Control

Jurkat Cell Slide(GTX25533) , Jurkat Cell Lysate(GTX27899)

Specificity/Sensitivity

At least two isoforms of TLR9 are known to exist; this antibody will detect both isoforms. TLR9 antibody is predicted to not cross-react with other TLR proteins.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

TLR9 antibody was raised against a peptide corresponding to 15 amino acids near the center of human TLR9.The immunogen is located within amino acids 840 - 890 of TLR9.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Toll Like Receptor 9,Cd289,Tlr9

Background

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX31295_894_IHC-P_20180221_18121410_163.jpg

GTX31295 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX31295 TLR9 antibody.
Working concentration : 10 μg/ml

GTX31295_2498_WB_20180221_18121410_577.jpg

GTX31295 WB Image

WB analysis of Jurkat cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX31295 TLR9 antibody.
Working concentration : 1 μg/ml

GTX31295_104_ICC-IF_20180221_18121410_678.jpg

GTX31295 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX31295 TLR9 antibody.
Working concentration : 2 μg/ml

Package List Price ($)
$ 329