APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5 - 1 μg/mL |
| 5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Human Lung Tissue Lysate(GTX27917) , Human Lung Tissue Slide
Product Note
At least three isoforms of TM4SF1 are known to exist; this antibody will recognize only the two largest isoforms. TM4SF1 antibody is predicted to not cross-react with other tetraspanin proteins.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
TM4SF1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human TM4SF1.The immunogen is located within the first 50 amino acids of TM4SF1.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
transmembrane 4 L six family member 1 , H-L6 , L6 , M3S1 , TAAL6
Cellular Localization
Membrane; Multi-pass membrane protein
Background
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface antigen and is highly expressed in different carcinomas. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX31430 WB Image
WB analysis of human lung tissue lysate using GTX31430 TM4SF1 antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml
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GTX31430 IHC-P Image
IHC-P analysis of mouse lung tissue using GTX31430 TM4SF1 antibody.
Dilution : 2 μg/ml
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GTX31430 IHC-P Image
IHC-P analysis of mouse lung tissue using GTX31430 TM4SF1 antibody.
Working concentration : 5 μg/ml
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GTX31430 IHC-P Image
IHC-P analysis of human lung tissue using GTX31430 TM4SF1 antibody.
Working concentration : 20 μg/ml
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GTX31430 IHC-P Image
IHC-P analysis of human lung tissue using GTX31430 TM4SF1 antibody.
Working concentration : 5 μg/ml
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GTX31430 IHC-P Image
IHC-P analysis of mouse lung tissue using GTX31430 TM4SF1 antibody.
Red : Primary antibody
Blue : DAPI
Dilution : 20 μg/ml
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REFERENCE
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REVIEW
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