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TMB Substrate

Cat No. GTX30945

Application

ELISA
Package
50 ml ($89)
PRODUCT

Summary

TMB Substrate

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ELISA Assay dependent
Not tested in other applications.

PROPERTIES

Storage

2-8ºC

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

3,3'5,5’- tetramethylbenzidine (TMB) is a soluble chromogen substrate for horseradish peroxidase detection systems for ELISA. This is ready to use one component system and gives soluble blue color with peroxidase which absorbs at 653 nm; on acidification gives stable yellow color that absorbs at 450 nm. The extinction coefficient of blue color ( E653 nm = 3.9 x 104 mol-1 cm-1 ), and the yellow color is ( E450 nm = 5.9 x 104 mol-1 cm-1 ). TMB yields stable blue color as end product ., that can be measured at 650 nm; the color formation is function of time The reaction is stopped by adding acid solution it yields a yellow color that can be measured at 450 nm. This yellow color is 1.5 X more sensitive than blue color. This TMB solution contains TMB, 2.08 mMol L-1, Hydrogen Peroxide substrate, citrate buffer and non-toxic proprietary stabilizers.

REFERENCE

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REVIEW

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Package List Price ($)
$ 89