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TMED4 antibody [N1C3]

Anti-TMED4 antibody [N1C3] used in Western Blot (WB). GTX112712
Anti-TMED4 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX112712
Anti-TMED4 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112712

Cat No. GTX112712

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TMED4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620429

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

transmembrane p24 trafficking protein 4 , ERS25 , GMP25iso , HNLF , p24a3 , p24alpha3

Cellular Localization

Endoplasmic reticulum membrane; Single-pass type I membrane protein

Background

Involved in endoplasmic reticulum stress response. May play a role in the regulation of heat-shock response and apoptosis.

Database

Research Area

DATA IMAGES

Anti-TMED4 antibody [N1C3] used in Western Blot (WB). GTX112712

GTX112712 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TMED4 antibody [N1C3] (GTX112712) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TMED4 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX112712

GTX112712 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung SCC, using TMED4(GTX112712) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TMED4 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112712

GTX112712 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using TMED4(GTX112712) antibody at 1:200 dilution.

REFERENCE

There are currently no references for TMED4 antibody [N1C3] (GTX112712). Be the first to share your publications with this product.

REVIEW

TMED4 antibody [N1C3] Cat No. GTX112712

Rating ( Average 4 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 18-Feb-2022
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : Caco-2 cells
Blocking : 0.25% saponin + 5% normal donkey serum in PBS, 25°C, 0.05Hr
Fixation : 3% PFA in PBS
Permeabilization /Antigen retrieval : 0.25% saponin + 5% normal donkey serum in PBS
Primary Antibody : 1:200, 37°C, 1Hr
Lane Description : Golgi-like perinuclear signals are evident in most of the cells.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169