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TMED4 antibody [N1C3]

Anti-TMED4 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX112712
Anti-TMED4 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112712
Anti-TMED4 antibody [N1C3] used in Western Blot (WB). GTX112712
Anti-TMED4 antibody [N1C3] used in IHC (Whole mount) (IHC-Wm). GTX112712

Cat. No. GTX112712

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TMED4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620429

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transmembrane p24 trafficking protein 4 , ERS25 , GMP25iso , HNLF , p24a3 , p24alpha3

Cellular Localization

Endoplasmic reticulum membrane; Single-pass type I membrane protein

Background

Involved in endoplasmic reticulum stress response. May play a role in the regulation of heat-shock response and apoptosis.

Database

Research Area

DATA IMAGES

Anti-TMED4 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX112712

GTX112712 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung SCC, using TMED4(GTX112712) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TMED4 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112712

GTX112712 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using TMED4(GTX112712) antibody at 1:200 dilution.

Anti-TMED4 antibody [N1C3] used in Western Blot (WB). GTX112712

GTX112712 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TMED4 antibody [N1C3] (GTX112712) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TMED4 antibody [N1C3] used in IHC (Whole mount) (IHC-Wm). GTX112712

GTX112712 IHC-Wm Image

TMED4 antibody [N1C3] detects Tmed4 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
TMED4 antibody [N1C3] (GTX112712) dilution: 1:50.

REFERENCE

There are currently no references for TMED4 antibody [N1C3] (GTX112712). Be the first to share your publications with this product.

REVIEW

TMED4 antibody [N1C3] Cat. No. GTX112712

Rating ( Average 4 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 18-Feb-2022
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : Caco-2 cells
Blocking : 0.25% saponin + 5% normal donkey serum in PBS, 25°C, 0.05Hr
Fixation : 3% PFA in PBS
Permeabilization /Antigen retrieval : 0.25% saponin + 5% normal donkey serum in PBS
Primary Antibody : 1:200, 37°C, 1Hr
Lane Description : Golgi-like perinuclear signals are evident in most of the cells.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169