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TMED9 antibody [N1C3]

Orthogonal
Anti-TMED9 antibody [N1C3] used in Western Blot (WB). GTX102163
Anti-TMED9 antibody [N1C3] used in Western Blot (WB). GTX102163
Anti-TMED9 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX102163
Anti-TMED9 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX102163

Cat. No. GTX102163

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all TMED9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

MCF-7

Predict Reactivity

Rat, Zebrafish, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TMED9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038138

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transmembrane p24 trafficking protein 9 , GMP25 , HSGP25L2G , p24a2 , p24alpha2 , p25

Cellular Localization

Endoplasmic reticulum membrane; Single-pass type I membrane protein

Database

Research Area

DATA IMAGES

Anti-TMED9 antibody [N1C3] used in Western Blot (WB). GTX102163

GTX102163 WB Image

Orthogonal

MCF-7 whole cell and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TMED9 antibody [N1C3] (GTX102163) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TMED9 antibody [N1C3] used in Western Blot (WB). GTX102163

GTX102163 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with TMED9 antibody [N1C3] (GTX102163) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TMED9 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX102163

GTX102163 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft, using TMED9(GTX102163) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TMED9 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX102163

GTX102163 IHC-P Image

TMED9 antibody [N1C3] detects TMED9 protein at cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
TMED9 antibody [N1C3] (GTX102163) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Yang YC et al. J Biomed Sci 2021; 28 (1) : 29 Proteomics-based identification of TMED9 is linked to vascular invasion and poor prognoses in patients with hepatocellular carcinoma.
Application : WB, IHC-P
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169