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TMEM119 antibody

Anti-TMEM119 antibody used in IHC (Frozen sections) (IHC-Fr). GTX134087
Anti-TMEM119 antibody used in IHC (Frozen sections) (IHC-Fr). GTX134087
Anti-TMEM119 antibody used in IHC (Frozen sections) (IHC-Fr). GTX134087
Anti-TMEM119 antibody used in IHC (Paraffin sections) (IHC-P). GTX134087
Anti-TMEM119 antibody used in IHC (Frozen sections) (IHC-Fr). GTX134087
Anti-TMEM119 antibody used in IHC (Paraffin sections) (IHC-P). GTX134087
Anti-TMEM119 antibody used in IHC (Paraffin sections) (IHC-P). GTX134087

Cat. No. GTX134087

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

IHC-P, IHC-Fr

Reactivity

Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.36 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the center region of mouse TMEM119. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887209

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transmembrane protein 119 , AW208946 , BC025600 , obif

Cellular Localization

Cell membrane,Cytoplasm,Endoplasmic reticulum membrane

Database

Research Area

DATA IMAGES

Anti-TMEM119 antibody used in IHC (Frozen sections) (IHC-Fr). GTX134087

GTX134087 IHC-Fr Image

TMEM119 antibody detects TMEM119 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse brain.
Red: TMEM119 stained by TMEM119 antibody (GTX134087) diluted at 1:250.
Green: Iba1 (isoform 1 and 3), stained by Iba1 (isoform 1 and 3) antibody, C-term (GTX89792) diluted at 1:250.
Blue: Hoechst 33342 staining.

Anti-TMEM119 antibody used in IHC (Frozen sections) (IHC-Fr). GTX134087

GTX134087 IHC-Fr Image

TMEM119 antibody detects TMEM119 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse brain.
Red: TMEM119 stained by TMEM119 antibody (GTX134087) diluted at 1:250.
Green: Iba1 (isoform 1 and 3), stained by Iba1 (isoform 1 and 3) antibody, C-term (GTX89792) diluted at 1:250.
Blue: Hoechst 33342 staining.

Anti-TMEM119 antibody used in IHC (Frozen sections) (IHC-Fr). GTX134087

GTX134087 IHC-Fr Image

TMEM119 antibody detects TMEM119 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse brain.
Red: TMEM119 stained by TMEM119 antibody (GTX134087) diluted at 1:250.
Green: Iba1 (isoform 1 and 3), stained by Iba1 (isoform 1 and 3) antibody, C-term (GTX89792) diluted at 1:250.
Blue: Hoechst 33342 staining.

Anti-TMEM119 antibody used in IHC (Paraffin sections) (IHC-P). GTX134087

GTX134087 IHC-P Image

TMEM119 antibody detects TMEM119 protein at microglia cells by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
TMEM119 stained by TMEM119 antibody (GTX134087) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TMEM119 antibody used in IHC (Frozen sections) (IHC-Fr). GTX134087

GTX134087 IHC-Fr Image

TMEM119 antibody detects TMEM119 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: TMEM119 stained by TMEM119 antibody (GTX134087) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-TMEM119 antibody used in IHC (Paraffin sections) (IHC-P). GTX134087

GTX134087 IHC-P Image

TMEM119 antibody detects TMEM119 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
TMEM119 stained by TMEM119 antibody (GTX134087) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TMEM119 antibody used in IHC (Paraffin sections) (IHC-P). GTX134087

GTX134087 IHC-P Image

TMEM119 antibody detects TMEM119 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
TMEM119 stained by TMEM119 antibody (GTX134087) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

TMEM119 antibody Cat. No. GTX134087

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 20-Oct-2022
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Mouse brain tissue section, 6μm
Blocking : Blocking ONE Histo (Nacalai), 25°C, 1Hr
Fixation : 4% PFA in 0.1M PBS
Permeabilization /Antigen retrieval : 0.1M PBS in 0.1% Tween20 / 0.01M Citrate Buffer, pH6.0, 95°C, 0.3Hr
Primary Antibody : 1:20, 4°C, 15Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169