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TMEM119 antibody

Anti-TMEM119 antibody used in IHC (Frozen sections) (IHC-Fr). GTX134087
Anti-TMEM119 antibody used in IHC (Frozen sections) (IHC-Fr). GTX134087
Anti-TMEM119 antibody used in IHC (Frozen sections) (IHC-Fr). GTX134087
Anti-TMEM119 antibody used in IHC (Paraffin sections) (IHC-P). GTX134087
Anti-TMEM119 antibody used in IHC (Paraffin sections) (IHC-P). GTX134087
Anti-TMEM119 antibody used in IHC (Frozen sections) (IHC-Fr). GTX134087
Anti-TMEM119 antibody used in IHC (Paraffin sections) (IHC-P). GTX134087

Cat No. GTX134087

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

IHC-P, IHC-Fr

Reactivity

Mouse
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.04 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the center region of mouse TMEM119. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887209

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

transmembrane protein 119 , AW208946 , BC025600 , obif

Cellular Localization

Cell membrane,Cytoplasm,Endoplasmic reticulum membrane

Database

Research Area

DATA IMAGES

Anti-TMEM119 antibody used in IHC (Frozen sections) (IHC-Fr). GTX134087

GTX134087 IHC-Fr Image

TMEM119 antibody detects TMEM119 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse brain.
Red: TMEM119 stained by TMEM119 antibody (GTX134087) diluted at 1:250.
Green: Iba1 (isoform 1 and 3), stained by Iba1 (isoform 1 and 3) antibody, C-term (GTX89792) diluted at 1:250.
Blue: Hoechst 33342 staining.

Anti-TMEM119 antibody used in IHC (Frozen sections) (IHC-Fr). GTX134087

GTX134087 IHC-Fr Image

TMEM119 antibody detects TMEM119 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse brain.
Red: TMEM119 stained by TMEM119 antibody (GTX134087) diluted at 1:250.
Green: Iba1 (isoform 1 and 3), stained by Iba1 (isoform 1 and 3) antibody, C-term (GTX89792) diluted at 1:250.
Blue: Hoechst 33342 staining.

Anti-TMEM119 antibody used in IHC (Frozen sections) (IHC-Fr). GTX134087

GTX134087 IHC-Fr Image

TMEM119 antibody detects TMEM119 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse brain.
Red: TMEM119 stained by TMEM119 antibody (GTX134087) diluted at 1:250.
Green: Iba1 (isoform 1 and 3), stained by Iba1 (isoform 1 and 3) antibody, C-term (GTX89792) diluted at 1:250.
Blue: Hoechst 33342 staining.

Anti-TMEM119 antibody used in IHC (Paraffin sections) (IHC-P). GTX134087

GTX134087 IHC-P Image

TMEM119 antibody detects TMEM119 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
TMEM119 stained by TMEM119 antibody (GTX134087) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TMEM119 antibody used in IHC (Paraffin sections) (IHC-P). GTX134087

GTX134087 IHC-P Image

TMEM119 antibody detects TMEM119 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
TMEM119 stained by TMEM119 antibody (GTX134087) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TMEM119 antibody used in IHC (Frozen sections) (IHC-Fr). GTX134087

GTX134087 IHC-Fr Image

TMEM119 antibody detects TMEM119 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: TMEM119 stained by TMEM119 antibody (GTX134087) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-TMEM119 antibody used in IHC (Paraffin sections) (IHC-P). GTX134087

GTX134087 IHC-P Image

TMEM119 antibody detects TMEM119 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
TMEM119 stained by TMEM119 antibody (GTX134087) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

TMEM119 antibody Cat No. GTX134087

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 20-Oct-2022
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Mouse brain tissue section, 6μm
Blocking : Blocking ONE Histo (Nacalai), 25°C, 1Hr
Fixation : 4% PFA in 0.1M PBS
Permeabilization /Antigen retrieval : 0.1M PBS in 0.1% Tween20 / 0.01M Citrate Buffer, pH6.0, 95°C, 0.3Hr
Primary Antibody : 1:20, 4°C, 15Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169