Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 0.5-1ug/ml |
| 0.25-0.5ug/ml for 30 minutes at RT |
|
Note :
IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
|
Not tested in other applications.
Calculated MW
Specificity/Sensitivity
Expression of DOG-1 protein is elevated in the gastrointestinal stromal tumors (GISTs), c-kit signaling-driven mesenchymal tumors of the GI tract. DOG-1 is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for DOG-1 has been reported in 97.8 percent of scorable GISTs, including all c-kit negative GISTs. Overexpression of DOG-1 has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha mutants that fail to express c-kit antigen. The overall sensitivity of DOG1 and c-kit in GISTs is nearly identical: 94.4% vs. 94.7%.
Form
Liquid
Buffer
Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Recombinant human DOG-1 protein
Purification
Ab purified from Bioreactor Concentrate by Protein A/G
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
![Anti-TMEM16A antibody [DG1/447] used in IHC (Paraffin sections) (IHC-P). GTX35125](/upload/website/prouct_img/small/GTX35125/GTX35125_20170712_IHC-P_18121410_775.jpg "Anti-TMEM16A antibody [DG1/447] used in IHC (Paraffin sections) (IHC-P). GTX35125")
![Anti-TMEM16A antibody [DG1/447] used in IHC (Paraffin sections) (IHC-P). GTX35125](/upload/website/prouct_img/normal/GTX35125/GTX35125_20170712_IHC-P_18121410_775.jpg "Anti-TMEM16A antibody [DG1/447] used in IHC (Paraffin sections) (IHC-P). GTX35125")
