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TMEM185A antibody

Anti-TMEM185A antibody used in Western Blot (WB). GTX87650
Anti-TMEM185A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87650

Cat. No. GTX87650

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μg ($399)
See all TMEM185A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
ICC/IF 1:100~1:500
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human TMEM185A (290-339).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CXorf13 , FAM11A , FRAXF , TMEM185A , ee3 , transmembrane protein 185A

Cellular Localization

Cell projection,Dendrite,Membrane

Background

The protein encoded by this gene is predicted to be a transmembrane protein. This gene is best known for localizing to the CpG island of the fragile site FRAXF. The 5' untranslated region of this gene contains a CGG trinucleotide repeat sequence that normally consists of 7-40 tandem CGG repeats but which can expand to greater than 300 repeats. Methylation of the CpG island leads to transcriptional silencing of this gene, but neither the silencing nor an expanded repeat region appear to manifest itself in a clear phenotypic manner. Alternative splicing results in multiple transcript variants. A pseudogene of this gene has been defined on the X chromosome. [provided by RefSeq, Aug 2013]

Database

Research Area

DATA IMAGES

Anti-TMEM185A antibody used in Western Blot (WB). GTX87650

GTX87650 WB Image

WB analysis of K562 cell lysates using GTX87650 TMEM185A antibody. The lane on the right is blocked with the synthesized peptide.

Anti-TMEM185A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87650

GTX87650 ICC/IF Image

ICC/IF analysis of MCF7 cells using GTX87650 TMEM185A antibody. The picture on the right is blocked with the synthesized peptide.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399