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TMEM59 antibody

Anti-TMEM59 antibody used in IHC (Paraffin sections) (IHC-P). GTX104486
Anti-TMEM59 antibody used in Western Blot (WB). GTX104486
Anti-TMEM59 antibody used in IHC (Paraffin sections) (IHC-P). GTX104486
Anti-TMEM59 antibody used in Western Blot (WB). GTX104486
Anti-TMEM59 antibody used in Western Blot (WB). GTX104486

Cat. No. GTX104486

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

A431 , Mouse liver , rat kidney

Predict Reactivity

Bovine, Chicken, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TMEM59. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952289

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transmembrane protein 59 , C1orf8 , DCF1 , HSPC001 , PRO195 , UNQ169

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Modulates the O-glycosylation and complex N-glycosylation steps occurring during the Golgi maturation of several proteins such as APP, BACE1, SEAP or PRNP. Inhibits APP transport to the cell surface and further shedding.

Database

Research Area

DATA IMAGES

Anti-TMEM59 antibody used in IHC (Paraffin sections) (IHC-P). GTX104486

GTX104486 IHC-P Image

TMEM59 antibody detects TMEM59 protein at cytosol on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
TMEM59 antibody (GTX104486) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TMEM59 antibody used in Western Blot (WB). GTX104486

GTX104486 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX104486 diluted at 1:1000

Anti-TMEM59 antibody used in IHC (Paraffin sections) (IHC-P). GTX104486

GTX104486 IHC-P Image

TMEM59 antibody detects TMEM59 protein at on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
TMEM59 antibody (GTX104486) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TMEM59 antibody used in Western Blot (WB). GTX104486

GTX104486 WB Image

TMEM59 antibody detects TMEM59 protein by Western blot analysis.
A. 50 μg rat kidney lysate/extract
10 % SDS-PAGE
TMEM59 antibody (GTX104486) dilution: 1:1000

Anti-TMEM59 antibody used in Western Blot (WB). GTX104486

GTX104486 WB Image

Sample(30 μg of whole cell lysate)
A:A431(GTX27909)
12% SDS PAGE
GTX104486 diluted at 1:500

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169