GeneTex
United States (US)

TMEM59 antibody

GTX104486_39911_WB_R_kidney_19011506_332.jpg
GTX104486_39911_IHC_19011506_131.jpg
GTX104486_WB_19011506_180.jpg
GTX104486_39911_IHC_M_19011506_885.jpg
GTX104486_39911_WB_M_liver_19011506_787.jpg

Cat No. GTX104486

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

A431 , mouse liver , rat kidney

Predict Reactivity

Bovine, Chicken, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TMEM59. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

C1orf8 antibody, FLJ42977 antibody, HSPC001 antibody, TMEM59 antibody, liver membrane-bound protein antibody, transmembrane protein 59 antibody

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Modulates the O-glycosylation and complex N-glycosylation steps occurring during the Golgi maturation of several proteins such as APP, BACE1, SEAP or PRNP. Inhibits APP transport to the cell surface and further shedding.

Database

Research Area

DATA IMAGES
GTX104486_39911_WB_R_kidney_19011506_332.jpg

GTX104486 WB Image

TMEM59 antibody detects TMEM59 protein by Western blot analysis.
A. 50 µg rat kidney lysate/extract
10 % SDS-PAGE
TMEM59 antibody (GTX104486) dilution: 1:1000

GTX104486_39911_IHC_19011506_131.jpg

GTX104486 IHC-P Image

TMEM59 antibody detects TMEM59 protein at cytosol on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
TMEM59 antibody (GTX104486) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104486_WB_19011506_180.jpg

GTX104486 WB Image

Sample(30 μg of whole cell lysate)
A:A431(GTX27909)
12% SDS PAGE
GTX104486 diluted at 1:500

GTX104486_39911_IHC_M_19011506_885.jpg

GTX104486 IHC-P Image

TMEM59 antibody detects TMEM59 protein at on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
TMEM59 antibody (GTX104486) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104486_39911_WB_M_liver_19011506_787.jpg

GTX104486 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX104486 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf