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TMEM66 antibody

Anti-TMEM66 antibody used in IHC (Paraffin sections) (IHC-P). GTX119858
Anti-TMEM66 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119858
Anti-TMEM66 antibody used in Western Blot (WB). GTX119858

Cat. No. GTX119858

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TMEM66 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TMEM66. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10731365

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

store-operated calcium entry associated regulatory factor , FOAP-7 , HSPC035 , TMEM66 , XTP3

Cellular Localization

Membrane; Multi-pass membrane protein

Database

Research Area

DATA IMAGES

Anti-TMEM66 antibody used in IHC (Paraffin sections) (IHC-P). GTX119858

GTX119858 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using Transmembrane protein 66(GTX119858) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TMEM66 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119858

GTX119858 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Transmembrane protein 66(GTX119858) antibody at 1:500 dilution.

Anti-TMEM66 antibody used in Western Blot (WB). GTX119858

GTX119858 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TMEM66 antibody (GTX119858) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169