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TMF1 antibody

Anti-TMF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX118431
Anti-TMF1 antibody used in Western Blot (WB). GTX118431
Anti-TMF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118431
Anti-TMF1 antibody used in Western Blot (WB). GTX118431
Anti-TMF1 antibody used in Western Blot (WB). GTX118431
Anti-TMF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX118431

Cat. No. GTX118431

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all TMF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

123 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse testis , rat testis

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TMF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885436

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TATA element modulatory factor 1 , ARA160 , TMF

Cellular Localization

Cytoplasm , Nucleus , Golgi apparatus membrane

Database

Research Area

DATA IMAGES

Anti-TMF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX118431

GTX118431 IHC-P Image

TMF1 antibody detects TMF1 protein at golgi on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
TMF1 antibody (GTX118431) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TMF1 antibody used in Western Blot (WB). GTX118431

GTX118431 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with TMF1 antibody (GTX118431) diluted at 1:500.

Anti-TMF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118431

GTX118431 ICC/IF Image

TMF1 antibody detects TMF1 protein at Golgi apparatus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: TMF1 protein stained by TMF1 antibody (GTX118431) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-TMF1 antibody used in Western Blot (WB). GTX118431

GTX118431 WB Image

TMF1 antibody detects TMF1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
5 % SDS-PAGE
TMF1 antibody (GTX118431) dilution: 1:1000

Anti-TMF1 antibody used in Western Blot (WB). GTX118431

GTX118431 WB Image

Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with TMF1 antibody (GTX118431) diluted at 1:500.

Anti-TMF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX118431

GTX118431 IHC-P Image

TMF1 antibody detects TMF1 protein at golgi on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
TMF1 antibody (GTX118431) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169