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TMF1 antibody

Anti-TMF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX118432
Anti-TMF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX118432
Anti-TMF1 antibody used in Western Blot (WB). GTX118432
Anti-TMF1 antibody used in Western Blot (WB). GTX118432
Anti-TMF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118432

Cat. No. GTX118432

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all TMF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:5000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

123 kDa. ( Note )

Positive Control

U87-MG , mouse testis

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TMF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177685

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TATA element modulatory factor 1 , ARA160 , TMF

Cellular Localization

Cytoplasm , Nucleus , Golgi apparatus membrane

Database

Research Area

DATA IMAGES

Anti-TMF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX118432

GTX118432 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using TMF1(GTX118432) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TMF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX118432

GTX118432 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using TMF1(GTX118432) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TMF1 antibody used in Western Blot (WB). GTX118432

GTX118432 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
5% SDS PAGE
GTX118432 diluted at 1:5000

Anti-TMF1 antibody used in Western Blot (WB). GTX118432

GTX118432 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with TMF1 antibody (GTX118432) diluted at 1:1000.

Anti-TMF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118432

GTX118432 ICC/IF Image

TMF1 antibody detects TMF1 protein at Golgi apparatus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TMF1 protein stained by TMF1 antibody (GTX118432) diluted at 1:5000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169