GeneTex
United States (US)

TMP21 antibody

GTX115517_41346_20141128_WB_M_18121410_271.jpg
GTX115517_41346_IHC_R_18121410_318.jpg
GTX115517_41346_20141128_WB_18121410_597.jpg
GTX115517_41346_20141128_WB_R_18121410_278.jpg

Cat No. GTX115517

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

293T , HepG2 , Neuro2A , CD830 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2

Predict Reactivity

Human, Mouse, Zebrafish, Rabbit, Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TMP21. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Transmembrane P24 Trafficking Protein 10,P24(Delta),S31I125,S31Iii125,Tmp21,Tmp-21-I,P23,P24D1,Tmed10

Cellular Localization

Golgi apparatus membrane; Single-pass type I membrane protein; Lumenal side , Melanosome

Background

This gene is a member of the EMP24/GP25L/p24 family and encodes a protein with a GOLD domain. This type I membrane protein is localized to the plasma membrane and golgi cisternae and is involved in vesicular protein trafficking. The protein is also a member of a heteromeric secretase complex and regulates the complex's gamma-secretase activity without affecting its epsilon-secretase activity. Mutations in this gene have been associated with early-onset familial Alzheimer's disease. This gene has a pseudogene on chromosome 8. [provided by RefSeq]

Database

DATA IMAGES
GTX115517_41346_20141128_WB_M_18121410_271.jpg

GTX115517 WB Image

TMP21 antibody detects TMP21 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TMP21 antibody (GTX115517) diluted by 1:1000.

GTX115517_41346_IHC_R_18121410_318.jpg

GTX115517 IHC-P Image

TMP21 antibody detects TMP21 protein at cytosol on rat brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain stem.
TMP21 antibody (GTX115517) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115517_41346_20141128_WB_18121410_597.jpg

GTX115517 WB Image

TMP21 antibody detects TMP21 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TMP21 antibody (GTX115517) diluted by 1:1000.

GTX115517_41346_20141128_WB_R_18121410_278.jpg

GTX115517 WB Image

TMP21 antibody detects TMP21 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TMP21 antibody (GTX115517) diluted by 1:1000.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169