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TMPRSS2 antibody [HL2229]

Recombinant antibody Orthogonal
Anti-TMPRSS2 antibody [HL2229] used in Western Blot (WB). GTX638274
Anti-TMPRSS2 antibody [HL2229] used in IHC (Paraffin sections) (IHC-P). GTX638274
Anti-TMPRSS2 antibody [HL2229] used in IHC (Paraffin sections) (IHC-P). GTX638274

Cat. No. GTX638274

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2229

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419),
25 μl ($169)
See all TMPRSS2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Product Note

This antibody was raised against human TMPRSS2 Extracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human TMPRSS2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transmembrane serine protease 2 , PP9284 , PRSS10

Cellular Localization

Cell membrane

Background

This gene encodes a protein that belongs to the serine protease family. The encoded protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. This gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Database

Research Area

DATA IMAGES

Anti-TMPRSS2 antibody [HL2229] used in Western Blot (WB). GTX638274

GTX638274 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TMPRSS2 antibody [HL2229] (GTX638274) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-TMPRSS2 antibody [HL2229] used in IHC (Paraffin sections) (IHC-P). GTX638274

GTX638274 IHC-P Image

TMPRSS2 antibody [HL2229] detects TMPRSS2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
TMPRSS2 stained by TMPRSS2 antibody [HL2229] (GTX638274) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TMPRSS2 antibody [HL2229] used in IHC (Paraffin sections) (IHC-P). GTX638274

GTX638274 IHC-P Image

TMPRSS2 antibody [HL2229] detects TMPRSS2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
TMPRSS2 stained by TMPRSS2 antibody [HL2229] (GTX638274) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 419
$ 169
Custom Conjugations