GeneTex
United States (US)

TMS1 antibody

TMS1-antibody_WB_GTX28394-1_18121411_770.jpg
TMS1-antibody_ICCIF_GTX28394-1_18121411_242.jpg

Cat No. GTX28394

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

Western blot at 1 μg/ml.

Calculated MW

22 kDa. ( Note )

Positive Control

HL60 whole cell lysate, an approximately 25 kDa band can be detected.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: RESQSYLVEDLERS, corresponding to amino acids 182-195 of Human ASC.

Purification

IgG fraction

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Pyd And Card Domain Containing,Asc,Card5,Tms,Tms-1,Tms1,Pycard

Cellular Localization

Cell Membrane. Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appeared as hollow, perinuclear spherical, ball-like structures.

Background

Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK, BCL10, Apaf-1, aRC, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified in human and mouse and designated ASC and TMS1 (1-3). Ectopic expression of ASC/TMS1 induced Apoptosis through activation of caspase-9 and inhibited the survival of human breast cancer cells (3,4). Overexpression of ASC/TMS1induced DNA fragmentation (4). ASC/TMS1 is expressed in a variety of human and mouse tissues (1, 2)

Database

Research Area

DATA IMAGES
TMS1-antibody_WB_GTX28394-1_18121411_770.jpg

GTX28394 WB Image

Western blot analysis of ASC in HL60 whole cell lysate in the absence (A) or presence (B) of blocking peptide with Anti-ASC (Cat. No. GTX28394) at 1 μg/mL.

TMS1-antibody_ICCIF_GTX28394-1_18121411_242.jpg

GTX28394 ICC/IF Image

Immunocytochemistry of TMS1 in HL60 cells with TMS1 antibody (GTX28394) at 5μg/ml.

Package List Price ($)
$ 329