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TMS1 antibody

Orthogonal
Anti-TMS1 antibody used in Western Blot (WB). GTX55818
Anti-TMS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55818
Anti-TMS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55818
Anti-TMS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55818
Anti-TMS1 antibody used in Western Blot (WB). GTX55818
Anti-TMS1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX55818

Cat. No. GTX55818

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all TMS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 50-195 of human PYCARD (NP_037390.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887938

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

PYD and CARD domain containing , ASC , CARD5 , TMS , TMS-1 , TMS1

Cellular Localization

Cytoplasm,Endoplasmic reticulum,Mitochondrion,Nucleus

Background

This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TMS1 antibody used in Western Blot (WB). GTX55818

GTX55818 WB Image

WB analysis of mouse spleen tissue lysate using GTX55818 TMS1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-TMS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55818

GTX55818 IHC-P Image

IHC-P analysis of rat spleen tissue using GTX55818 TMS1 antibody.
Dilution : 1:250
Blue : DAPI

Anti-TMS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55818

GTX55818 ICC/IF Image

ICC/IF analysis of A549 cells using GTX55818 TMS1 antibody.
Dilution : 1:250

Anti-TMS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55818

GTX55818 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX55818 TMS1 antibody.
Dilution : 1:250
Blue : DAPI

Anti-TMS1 antibody used in Western Blot (WB). GTX55818

GTX55818 WB Image

WB analysis of various cell lysates using GTX55818 TMS1 antibody.
Dilution : 1:1000
Loading : 25μg

Anti-TMS1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX55818

GTX55818 IHC-Fr Image

Orthogonal

The data was published in the journal Sci Rep in 2018. PMID: 29434214

REFERENCE

Application Reference

Gu X et al. Exp Ther Med 2023; 26 (3) : 413 1,25(OH)(2)D(3) ameliorates doxorubicin?induced cardiomyopathy by inhibiting the NLRP3 inflammasome and oxidative stress.
Li Z et al. Front Pharmacol 2023; 14 : 1224906 A novel colchicine-myricetin heterozygous molecule: design, synthesis, and effective evaluations on the pathological models of acute lung injury in vitro and in vivo.
XIN GU et al. Exp Ther Med 2023; 26 : 413 1,25(OH)?D? ameliorates doxorubicin?induced cardiomyopathy by inhibiting the NLRP3 inflammasome and oxidative stress
Application : WB
Liang Y et al. Oxid Med Cell Longev 2022; 2022 : 3415528 p16(INK4a) Plays Critical Role in Exacerbating Inflammaging in High Fat Diet Induced Skin.
Application : WB
Reactivity : Mouse
Kapka Miteva et al. Sci Rep. 2018; 8 (1) Mesenchymal stromal cells inhibit NLRP3 inflammasome activation in a model of Coxsackievirus B3-induced inflammatory cardiomyopathy
Application : IHC-Fr
Reactivity : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399