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TNF Receptor II antibody [80M2]

Cat. No. GTX54424

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 80M2

Isotype

 IgG1

Application

 WB, ICC/IF, FACS, IP, ELISA, Functional Assay

Reactivity

 Human, Rat
Package
50 μg ($399)
See all TNF Receptor II products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
IP Assay dependent
ELISA Assay dependent
Functional Assay Assay dependent

Note :

Functional Assay
Antibody 80M2 functions as an allosteric modulator, which stabilizes the ligand-receptor complex.
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Product Note

The monoclonal antibody 80M2 recognizes the extracellular part of membrane-bound TNF-RII as well as the soluble form of TNF-RII which is generated by proteolytic cleavage of the extracellular domain. The soluble form can still bind TNF-alpha with high affinity and functions as a TNF-alpha antagonist. The antibody is a non-agonistic receptor modulating antibody. It enhances in vitro TNF alpha responses by increasing the affinity of the soluble form of TNF-alpha for TNF-RII.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 0.1% BSA

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

100 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Purification

Purified IgG1

Conjugation

Unconjugated

RRID

AB_2887857

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD120b,TBPII,TNF receptor superfamily member 1B,TNFBR,TNFR1B,TNFR2,TNFR75,TNFR80,TNFRII,TNFRSF1B,p75,p75TNFR,TNF receptor II

Cellular Localization

Cell membrane,Secreted

Background

The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. The function of IAPs in TNF-receptor signalling is unknown, however, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. [provided by RefSeq, Jul 2008]

Database

Research Area

REFERENCE

Application Reference

Lee CJ et al. Cytokine 2019; 113 : 340-346 Development of an inflammatory tissue-selective chimeric TNF receptor.
Application : WB
Reactivity : Human
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Package List Price ($)
$ 399