APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Assay dependent |
10μg/ml antibody in PBS/1% serum / 10⁵ microglia cells |
Assay dependent |
Assay dependent |
2μg/ml |
Note :
WB
A reduced sample treatment and SDS-Page was used.
Activation
In most cases acts agonistically.
|
Not tested in other applications.
Calculated MW
Product Note
The monoclonal antibody HM102 recognizes the extracellular part of membrane-bound TNF-RII as well as the soluble form of TNF-RII which is generated by proteolytic cleavage of the extracellular domain. The soluble form can still bind TNF-alpha with high affinity and functions as a TNF-alpha antagonist. The antibody is a agonistic receptor modulating antibody. It enhances in vitro TNF alpha responses by increasing the affinity of the soluble form of TNF-alpha for TNF-RII.
PROPERTIES
Form
Liquid
Buffer
Filter-sterilized PBS, 0.1% BSA
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
100 μg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
The monoclonal antibody HM102 recognizes the extracellular part of membrane-bound TNF Receptor II as well as the soluble form of TNF Receptor II which is generated by proteolytic cleavage of the extracellular domain.
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD120b,TBPII,TNF receptor superfamily member 1B,TNFBR,TNFR1B,TNFR2,TNFR75,TNFR80,TNFRII,TNFRSF1B,p75,p75TNFR,TNF receptor II
Cellular Localization
Cell membrane,Secreted
Background
The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. The function of IAPs in TNF-receptor signalling is unknown, however, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. [provided by RefSeq, Jul 2008]
Database
Research Area
REFERENCE
There are currently no references for TNF Receptor II antibody [HM102] (GTX27369). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for TNF Receptor II antibody [HM102] (GTX27369). Be the first to share your experience with this product.