GeneTex
United States (US)

TNF alpha antibody [2TNF-H33] (Biotin)

GTX15792_70_ELISA_19011519_112.jpg

Cat No. GTX15792

Host Mouse
Clonality Monoclonal
Clone Name 2TNF-H33
Isotype IgG1
Application WB, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 4% BSA

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human TNF alpha

Purification

Protein G purified

Conjugation

Biotin

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tumor Necrosis Factor,Dif,Tnf-Alpha,Tnfa,Tnfsf2,Tnlg1F,Tnf

Cellular Localization

Cell membrane,Membrane,Secreted

Background

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX15792_70_ELISA_19011519_112.jpg

GTX15792 ELISA Image

Sandwich ELISA analysis of serial diluted recombinant human TNF alpha using GTX15792 TNF alpha antibody [2TNF-H33] (Biotin) (detection antibody) in duplicate at 2, 1, 0.5, and 0.25 µg/ml and rabbit anti-Human TNF (capture antibody) at 5 µg/ml.