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TNF alpha antibody [52B83]

Cat. No. GTX54419

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 52B83

Isotype

 IgG1

Application

 WB, IHC-P, IHC-Fr, FACS, ELISA

Reactivity

 Human, Mouse, Rat, Guinea pig, Rhesus Monkey
Package
50 μg ($399)
See all TNF alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
ELISA Assay dependent

Note :

WB
A reduced sample treatment and 15% SDS-Page was used.

IHC-Fr
6-μm tissue sections were fixed in acetone for 10 minutes.

FACS
Cells were fixed in PBA containing 0.2% formaldehyde.
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 0.1% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

100 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DIF,TNF,TNFA,TNFSF2,TNFalpha,TNLG1F,tumor necrosis factor,TNF alpha

Cellular Localization

Cell membrane,Membrane,Secreted

Background

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]

Database

Research Area

REFERENCE

Application Reference

Saclier M et al. J Pathol 2022; 257 (3) : 352-366 Selective ablation of Nfix in macrophages attenuates muscular dystrophy by inhibiting fibro-adipogenic progenitor-dependent fibrosis.
Reactivity : Mouse

Clone Reference

ASC Veras et al. et al. Sci Rep 2021; 11 (1):6282 Supplementation of polyunsaturated fatty acids (PUFAs) and aerobic exercise improve functioning, morphology, and redox balance in prostate obese rats
Application : IHC-P
Reactivity : Rat
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REVIEW

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Package List Price ($)
$ 399