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TNF alpha antibody [B-D9] (FITC)

Cat. No. GTX11457

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 B-D9

Isotype

 IgG1

Application

 FACS

Reactivity

 Human
Package
50 μg ($459)
See all TNF alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS Neat

Note :

FACS
Methanol treatment is required prior to antibody staining. Use 10μl of the suggested working dilution to label 5 x 10 5 cells in 50μl.
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Product Note

This antibody recognizes human TNF alpha, both natural and recombinant forms.

PROPERTIES

Form

Liquid

Buffer

PBS, 5% BSA

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human TNF-alpha.

Purification

Purified by ion exchange chromatography

Conjugation

Fluorescein isothiocyanate (FITC)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tumor necrosis factor , DIF , TNF-alpha , TNFA , TNFSF2 , TNLG1F

Cellular Localization

Cell membrane

Background

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]

Database

Research Area

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 459