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TNF alpha antibody (Biotin)

Cat. No. GTX17442

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Human
Package
25 μg ($399)
See all TNF alpha products

APPLICATION

Application Note

ELISA: Use at a concentration of 0.15 - 0.3 μg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant human TNF alphA. WB: Use at a concentration of 0.1 - 0.2 μg/ml. Predicted molecular weight: 26 kDa. Used in conjunction with compatible secondary reagents the detection limit for recombinant human TNF alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.1% BSA, PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.05 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Highly pure (>98%) recombinant human TNF alpha

Purification

Immunogen affinity purified

Conjugation

Biotin

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tumor necrosis factor , DIF , TNF-alpha , TNFA , TNFSF2 , TNLG1F

Cellular Localization

Cell membrane

Background

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]

Database

Research Area

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399