APPLICATION
Application Note
ELISA: Use at an assay dependent dilution. GTX18696 can be used as a capture antibody in sandwich ELISA applications for human TNF-alpha detection in combination with a monoclonal tracer/detection antibody (biotinconjugated). 4 pg/ml of TNF-alpha in serum/plasma or medium can be detected with an assay range of 0 to 2000 pg/ml. Suggested capture coating dose: 1.0-2.0μg/well IHC: Use at a concentration of 10 μg/ml. Reacts with formalin-fixed paraffin-embedded tissues at a monoclonal antibody concentration of 10μg/mL. Strong reaction with normal kidney, liver with cirrhosis, and normal lung samples, positive reaction with lung cancer samples.Neut: GTX18696 neutralizes TNF-alpha activity in vitro. Use 2-5 ng of GTX18696 per μg of MAb. Optimal dilutions/concentrations should be determined by the end user.
Calculated MW
Product Note
This monoclonal antibody recognizes both recombinant and native human TNF-alpha.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant full length protein (Human).
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
tumor necrosis factor , DIF , TNF-alpha , TNFA , TNFSF2 , TNLG1F
Cellular Localization
Cell membrane
Background
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]
Database
Research Area
REFERENCE
There are currently no references for TNF alpha antibody [CH8820] (GTX18696). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for TNF alpha antibody [CH8820] (GTX18696). Be the first to share your experience with this product.