Indirect ELISA analysis was performed by coating the plate with recombinant 293T expressed, human Fc- tagged TNF alpha(54-233 a.a.) (201.71-3.15 nM). Coated protein was probed with TNF alpha antibody [F6C5] (GTX28349) (1 μg/mL).
Please notice that GTX28349 needs to be conjugated to biotin to function as the detection antibody. Please contact us for custom biotin-conjugated antibody.